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Updated: Feb 5, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
Published on: March 20, 2020
Long intergenic noncoding RNA 299 methylation in peripheral blood is a biomarker for triple-negative breast cancer
Justo L Bermejo1, Guanmengqian Huang2, Mehdi Manoochehri2
1Statistical Genetics Group, Institute of Medical Biometry & Informatics, University of Heidelberg, Heidelberg, 69120, Germany.
Aim:
To identify DNA methylation biomarkers in peripheral blood samples from triple-negative breast cancer (TNBC) patients.
Materials & Methods:
We conducted an epigenome-wide association study (EWAS): the most promising markers were identified in 233 TNBC case-control pairs (discovery set) and subsequently validated in an independent validation set (57 TNBC patients and 124 controls).
Results:
cg06588802 (LINC00299/ID2) showed a higher methylation in TNBC patients compared with controls (discovery set: 3% increase, p-value = 0.0009; validation set: 2% increase, p-value = 0.01). Consistent results at four neighboring methylation probes and the strong negative correlation (rho = -0.93) with LINC00299 expression add plausibility to this result.
Conclusion:
Hypermethylation of LINC00299 in peripheral blood may constitute a useful circulating biomarker for TNBC.
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