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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
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Author Correction: Enhancer decommissioning by LSD1 during embryonic stem cell differentiation.

Warren A Whyte1,2, Steve Bilodeau1, David A Orlando1

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.

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|September 14, 2018
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Summary

This letter corrects western blot data for LSD1 and tubulin, ensuring accurate representation of protein levels. Amendments clarify lane juxtapositions and provide raw data for transparency in scientific findings.

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Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • Western blotting is a key technique for protein analysis.
  • Accurate data presentation is crucial for scientific integrity.

Purpose of the Study:

  • To correct inadvertent errors in western blot figures.
  • To ensure accurate representation of LSD1, tubulin, and HDAC1 protein levels.
  • To provide corrected figures and raw data for transparency.

Main Methods:

  • Western blotting was used to analyze protein expression.
  • Image analysis and figure preparation were performed.
  • Amendments included correcting duplicated images and delineating non-adjacent lanes.

Main Results:

  • An inadvertent duplication of a tubulin blot for LSD1 was identified and corrected.
  • Corrected western blots for LSD1, tubulin, and HDAC1 are presented.
  • Vertical lines were added to figures to clarify lane juxtapositions.

Conclusions:

  • The corrections ensure the accurate depiction of protein expression data.
  • The revised figures and supplementary data enhance the reliability of the study's findings.
  • Transparency in data presentation is maintained through the provision of raw images.