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Mesoporous silica coating NaYF

Sai Xu1, Yang Yu1, Yuefeng Gao2

  • 1College of Science, Dalian Maritime University, Dalian, 116026, Liaoning, People's Republic of China.

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|September 14, 2018
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Summary

New multifunctional nanocomposites enable simultaneous in-vitro imaging of temperature and oxygen concentration in cancer cells. This dual-mode sensing technology offers precise cellular-level diagnostics for tumor research.

Keywords:
Core-shell structureDual sensorHepG-2LuminophoreOptical sensorRare earth ionsThermal coupled levelsTumor diagnosis

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Materials Science

Background:

  • Accurate cellular-level detection of temperature and oxygen concentration is crucial for tumor diagnosis.
  • Existing methods may lack the specificity or multifunctionality required for comprehensive cellular analysis.

Purpose of the Study:

  • To develop and characterize multifunctional nanocomposites for simultaneous temperature and oxygen sensing.
  • To demonstrate the in-vitro application of these nanocomposites for dual-mode imaging in hepatocellular carcinoma cells.

Main Methods:

  • Synthesis of NaYF4:Yb,Er@NaYF4@mSiO2-Ru nanocomposites integrating upconversion nanoparticles, mesoporous silica, and a ruthenium complex.
  • Utilizing red downconversion luminescence (455/606 nm) for oxygen sensing and green upconversion luminescence (980/525 & 544 nm) for temperature measurement.
  • Applying the nanocomposites for dual-mode in-vitro imaging of temperature and oxygen in HepG-2 cells.

Main Results:

  • The nanocomposites exhibited distinct luminescence modes for oxygen sensing (oxygen-quenched red luminescence) and temperature measurement (green upconversion luminescence).
  • Successful dual-mode in-vitro imaging of temperature and oxygen concentration within HepG-2 cells was achieved.
  • The developed system demonstrated potential for precise cellular-level diagnostics.

Conclusions:

  • The multifunctional nanocomposites provide a novel platform for simultaneous, non-invasive monitoring of temperature and oxygen in biological systems.
  • This technology holds promise for advancing tumor diagnosis and understanding cellular microenvironments.
  • Further research can explore in-vivo applications and optimization for clinical settings.