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Tungsten disulfide (WS

Yunlei Zhou1, Chengji Sui1, Huanshun Yin2

  • 1College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, People's Republic of China.

Mikrochimica Acta
|September 14, 2018
PubMed
Summary

This study presents a new photoelectrochemical method for detecting chloramphenicol (CLOA) using WS2 nanosheets and aptamers. The method enhances sensitivity through DNase I-assisted target recycling, achieving a low detection limit.

Keywords:
AmperometryAntibiotic detectionDNA aptamerFood samplePhotoelectrochemistrySignal amplificationTransition metal dichalcogenideTungsten disulfide

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Area of Science:

  • Electrochemistry
  • Nanomaterials Science
  • Biotechnology

Background:

  • Chloramphenicol (CLOA) is an antibiotic with potential toxicity, necessitating sensitive detection methods.
  • Existing detection techniques may lack the required sensitivity, selectivity, or speed for real-world applications.
  • Photoelectrochemical methods offer advantages in sensitivity and simplicity for analyte detection.

Purpose of the Study:

  • To develop a novel, highly sensitive photoelectrochemical apta-biosensor for chloramphenicol (CLOA) detection.
  • To utilize tungsten disulfide (WS2) nanosheets as photoactive materials for enhanced signal transduction.
  • To incorporate a DNase I-assisted target recycling strategy to amplify the analytical signal.

Main Methods:

  • Fabrication of a photoelectrochemical apta-biosensor using WS2 nanosheets and DNA aptamers.
  • Employing aptamer adsorption on WS2 to modulate photocurrent in the absence of CLOA.
  • Implementing DNase I-catalyzed recycling of CLOA to amplify the photocurrent signal upon analyte binding.

Main Results:

  • The apta-biosensor demonstrated a linear response to CLOA in the concentration range of 10 pM to 10 nM.
  • A low limit of detection (LOD) of 3.6 pM was achieved under optimal conditions.
  • The method exhibited acceptable selectivity, accuracy, and stability, with successful application in spiked milk samples.

Conclusions:

  • The developed photoelectrochemical apta-biosensor provides a simple, sensitive, and reliable platform for CLOA determination.
  • The combination of WS2 nanosheets and DNase I-assisted recycling significantly enhances detection performance.
  • This approach holds promise for the rapid and accurate monitoring of chloramphenicol in food matrices.