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Updated: Feb 5, 2026

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients
07:43

Using 22C3 Anti-PD-L1 Antibody Concentrate on Biopsy and Cytology Samples from Non-small Cell Lung Cancer Patients

Published on: September 25, 2018

11.0K

Comparable immunoreactivity rates of PD-L1 in archival and recent specimens from non-small cell lung cancer

Yuki Nakamura1, Tetsu Kobayashi2, Yoichi Nishii1

  • 1Respiratory Center, Matsusaka Municipal Hospital, Matsusaka, Japan.

Thoracic Cancer
|September 14, 2018
PubMed
Abstract

Insights

The time between collecting and testing non-small cell lung cancer (NSCLC) tissue for PD-L1 expression does not impact immunohistochemistry results. This finding supports reliable use of archival samples for predicting response to immunotherapy.

Area of Science:

  • Oncology
  • Immunology
  • Pathology

Background:

  • Molecular targeted therapy, including immune checkpoint inhibitors (ICIs) like anti-PD-1/PD-L1 antibodies, has significantly improved cancer patient outcomes.
  • Tumor expression levels of Programmed Death-Ligand 1 (PD-L1) are crucial biomarkers for predicting response rates to ICIs.
  • Standardization of PD-L1 assessment is vital for clinical decision-making in non-small cell lung cancer (NSCLC).

Purpose of the Study:

  • To investigate the impact of the time interval between tumor tissue sampling/paraffinization and immunohistochemistry (IHC) on PD-L1 staining levels in NSCLC.
  • To determine if archival tissue samples yield comparable PD-L1 immunoreactivity to recent samples.
  • To assess the reliability of PD-L1 testing on stored NSCLC specimens for predicting immunotherapy response.

Main Methods:

  • A cohort of 137 NSCLC patients was analyzed.
  • Tumor tissues were stained for PD-L1 expression using 22C3 and 28-8 antibodies via immunohistochemistry.
  • Immunoreactivity rates were compared between archival and recent tissue samples, and correlated with various clinicopathological factors.

Main Results:

  • A significant correlation in PD-L1 immunoreactivity was observed between 22C3 and 28-8 antibody clones.
  • No statistically significant difference in PD-L1 immunoreactivity was found between archival and recent NSCLC tissue samples.
  • PD-L1 expression levels correlated with tumor histological type and size, but not with specimen storage time, age, gender, smoking history, clinical stage, or lymph node metastasis.

Conclusions:

  • The time interval between tissue sampling/paraffinization and IHC analysis does not influence PD-L1 immunoreactivity in NSCLC.
  • Archival NSCLC tissue samples are reliable for PD-L1 assessment, supporting their use in clinical practice and research.
  • These findings affirm the stability of PD-L1 expression over time in stored tissues, reinforcing its utility as a predictive biomarker for immunotherapy.
Keywords:
Archival specimenPD-L1immune checkpoint inhibitormolecular targeted therapynon-small cell lung cancer

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