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Updated: Feb 5, 2026

Fluorimetric Techniques for the Assessment of Sperm Membranes
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Comparison between electroporation and polyfection in pig sperm: efficiency and cell viability implications

Zigomar da Silva1, Andressa Pereira de Souza1, José Rodrigo Claudio Pandolfi2

  • 12Santa Catarina State University.College of Agriculture and Veterinary - CAV,Av. Luiz de Camões,2090,Conta Dinheiro,CEP: 88.520-000,Lages City,SC,Brazil.

Zygote (Cambridge, England)
|September 14, 2018
PubMed
Summary

Polyfection (PLF) using polyethyleneimine (PEI) is the most efficient method for swine sperm transfection, achieving 76.8% efficacy. Electroporation (EP) showed lower, but still improved, transfection rates compared to controls.

Keywords:
ElectroporationFlow cytometryPigPolyethyleneimineSperm viability

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