Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Quantitative Analysis01:12

Quantitative Analysis

1.4K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
1.4K
Quantitative Aspects of Drug-Receptor Interaction01:30

Quantitative Aspects of Drug-Receptor Interaction

1.8K
The receptor occupancy theory connects a drug's response to the number of occupied receptors. With higher drug concentrations, more receptors are occupied, leading to increased responses. The formation of drug-receptor complexes involves association and dissociation rates, which reach equilibrium when the forward and backward reactions are equal. The equilibrium association constant (Ka) and its inverse, the equilibrium dissociation constant (Kd), indicate drug affinity. Higher Ka and lower...
1.8K
Reaction Stoichiometry02:57

Reaction Stoichiometry

78.0K
A balanced chemical equation provides a great deal of information in a very succinct format. Chemical formulas provide the identities of the reactants and products involved in the chemical change, allowing classification of the reaction. Coefficients provide the relative numbers of these chemical species, allowing a quantitative assessment of the relationships between the amounts of substances consumed and produced by the reaction. These quantitative relationships are known as the reaction’s...
78.0K
The Scientific Method03:50

The Scientific Method

67.6K
Chemistry is an empirical science. Scientists often pose questions to understand the chemistry in everyday life and seek answers to these questions. To achieve this, scientists follow a definitive series of steps that together make up the Scientific Method. This approach involves making observations, asking questions, building a hypothesis, conducting experiments, analyzing results, and forming a conclusion. 
67.6K
Case Studies01:22

Case Studies

13.6K
There are many research methods available to psychologists in their efforts to understand, describe, and explain behavior and the cognitive and biological processes that underlie it.
13.6K
Relative Strengths of Conjugate Acid-Base Pairs02:29

Relative Strengths of Conjugate Acid-Base Pairs

52.6K
Brønsted-Lowry acid-base chemistry is the transfer of protons; thus, logic suggests a relation between the relative strengths of conjugate acid-base pairs. The strength of an acid or base is quantified in its ionization constant, Ka or Kb, which represents the extent of the acid or base ionization reaction. For the conjugate acid-base pair HA / A−, the ionization equilibrium equations and ionization constant expressions are
52.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

OCT-Defined Foveal Restoration Is Associated with Visual Recovery after Vitrectomy for Lamellar Macular Hole: A Multicenter Study: LMH Report No. 1.

Ophthalmology. Retina·2026
Same author

Cyclin-dependent kinase 10 controls bone formation and is linked to human skeletal health.

Journal of orthopaedic translation·2026
Same author

From pixels to pores: 3D-(im)printed hierarchically porous polymer monoliths.

iScience·2026
Same author

Pesticide residue analysis of edible insects: method development and market survey in Japan.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment·2025
Same author

Automated dual-cartridge solid-phase extraction method for multi-residue determination of veterinary drugs in bovine muscle, liver, fat, and milk.

Analytical methods : advancing methods and applications·2025
Same author

[Collaborative Study of Detecting Method for Foreign Amylase in Honey using Native Polyacrylamide Gel Electrophoresis-Activity Staining].

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·2025

Related Experiment Video

Updated: Feb 5, 2026

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods
08:28

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

Published on: March 10, 2020

7.4K

Quantitative

Tsuyoshi Kato1, Mami Nishimiya1, Akiko Kawata1

  • 1Japan Food Research Laboratories.

Journal of Oleo Science
|September 14, 2018
PubMed
Summary

This study presents an optimized 31P NMR method for accurately quantifying phospholipid classes in various samples. The method ensures precise, simultaneous measurement of major and minor phospholipids using an internal standard for reliable results.

Keywords:
phospholipidaccurate quantitative NMR with internal reference substance (AQARI)phosphorus nuclear magnetic resonancequantitative nuclear magnetic resonance

More Related Videos

Quantitative Autonomic Testing
11:40

Quantitative Autonomic Testing

Published on: July 19, 2011

58.6K
A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

15.0K

Related Experiment Videos

Last Updated: Feb 5, 2026

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods
08:28

Analysis of Congenital Heart Defects in Mouse Embryos Using Qualitative and Quantitative Histological Methods

Published on: March 10, 2020

7.4K
Quantitative Autonomic Testing
11:40

Quantitative Autonomic Testing

Published on: July 19, 2011

58.6K
A Quantitative Fitness Analysis Workflow
11:39

A Quantitative Fitness Analysis Workflow

Published on: August 13, 2012

15.0K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Nuclear Magnetic Resonance Spectroscopy

Background:

  • Accurate quantification of phospholipid classes is crucial for understanding biological processes and nutritional content.
  • Existing methods may lack precision or the ability to simultaneously quantify diverse phospholipid species.
  • Phospholipids are key components in biological membranes and dietary sources like lecithin.

Purpose of the Study:

  • To develop and validate an absolute quantification 31P NMR method for individual and concomitant analysis of phospholipid classes.
  • To optimize measurement conditions for simultaneous detection of major and minor phospholipids, including lysophospholipids.
  • To establish a method with traceability to the International System of Units (SI) for precise molar quantity determination.

Main Methods:

  • A modified 31P NMR method was employed using an anionic surfactant (sodium cholate) solution with EDTA.
  • Phospholipid standards and food samples were analyzed under controlled pH (6.90±0.04) and temperature (30.0±0.1°C).
  • Phosphoserine was used as an internal standard for absolute molar quantification, with subsequent translation to weight amounts.

Main Results:

  • The 31P NMR method demonstrated reproducible chemical shifts for each phospholipid class.
  • Quantification of phosphatidylcholine by 31P NMR showed strong agreement with 1H NMR.
  • Simultaneous quantification of major and minor phospholipids in commercial samples (porcine brain, yeast, soybean) was achieved.
  • Results from 31P NMR correlated well with molybdenum blue colorimetry for total phospholipid content.
  • 31P NMR proved more informative for separating individual phospholipid species compared to 2D thin-layer chromatography.

Conclusions:

  • The optimized 31P NMR method enables accurate and simultaneous absolute quantification of various phospholipid classes.
  • This technique provides a reliable and informative approach for analyzing complex lipid mixtures in diverse biological and food samples.
  • The method offers improved resolution for individual phospholipid species compared to traditional chromatographic techniques.