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Hongbo Yi1, Li Wang1, Yunxia Xiong1

  • 1State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, 1 Dafeng 1st Street, Guangzhou 510640, China.

Mediators of Inflammation
|September 14, 2018
PubMed
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Lactobacillus reuteri LR1 helps repair intestinal barrier damage caused by ETEC K88. This probiotic enhances tight junction proteins (ZO-1 and occludin) via the MLCK pathway, improving gut health.

Area of Science:

  • Microbiology
  • Immunology
  • Gastroenterology

Background:

  • Intestinal epithelial barrier damage contributes to immune dysregulation and various gut disorders.
  • Lactobacillus reuteri strains are recognized for their probiotic properties, influencing gut microbiota and immunity.

Purpose of the Study:

  • To investigate the effects of a novel Lactobacillus reuteri LR1 strain on intestinal epithelial barrier integrity.
  • To determine if L. reuteri LR1 can mitigate damage induced by enterotoxigenic Escherichia coli (ETEC) K88 in IPEC-1 cells.

Main Methods:

  • Utilized IPEC-1 cell monolayers challenged with ETEC K88.
  • Assessed cell monolayer permeability, bacterial adhesion, and invasion.
  • Quantified tight junction protein expression (ZO-1, occludin, claudin-1) via transcript and protein analysis.

Related Experiment Videos

  • Employed colloidal gold immunoelectron microscopy for protein localization.
  • Investigated the role of myosin light-chain kinase (MLCK) pathway using ML-7 inhibitor.

    • Main Results:

    • L. reuteri LR1 significantly reduced ETEC K88-induced permeability in IPEC-1 cells.
    • The probiotic decreased ETEC K88 adhesion and invasion of IPEC-1 cells.
    • L. reuteri LR1 upregulated ZO-1 and occludin expression, crucial for tight junctions.
    • Effects on ZO-1 and occludin were confirmed via immunoelectron microscopy and found to be MLCK-dependent.

    Conclusions:

    • Lactobacillus reuteri LR1 demonstrates significant protective effects on intestinal epithelial barrier function against ETEC K88 challenge.
    • The probiotic enhances barrier integrity by increasing ZO-1 and occludin expression through a MLCK-dependent mechanism.
    • L. reuteri LR1 holds potential as a probiotic intervention for managing intestinal disorders linked to barrier dysfunction.