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Global Transcriptomic Analysis and Function Identification of Malolactic Enzyme Pathway of

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Lactobacillus paracasei

Keywords:
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Area of Science:

  • Microbiology
  • Gut Microbiome Research
  • Probiotic Bacteria

Background:

  • Bile tolerance is essential for Lactobacillus paracasei survival in the gut.
  • Understanding stress response mechanisms is key to probiotic efficacy.

Purpose of the Study:

  • To investigate the molecular mechanisms of bile stress tolerance in Lactobacillus paracasei L9.
  • To identify genes and pathways involved in surviving bile exposure.

Main Methods:

  • Global transcriptomic analysis of L. paracasei L9 under ox-bile stress.
  • Genetic and physiological experiments to validate gene functions.
  • Analysis of malolactic enzyme (MLE) pathway involvement.

Main Results:

  • 104 genes were significantly altered under bile stress.
  • The malolactic enzyme (mleS) gene was upregulated 2.60-fold.
  • L-malic acid enhanced bile tolerance by promoting intracellular alkalinization and membrane balance.

Conclusions:

  • The malolactic enzyme pathway is crucial for Lactobacillus paracasei L9's bile stress response.
  • Maintaining membrane balance is a key mechanism for surviving bile stress.
  • This study provides insights into the molecular basis of bile tolerance in lactic acid bacteria.