Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

18.5K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.5K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.3K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

P2X3 receptor-mediated the enhanced interaction between sensitized carotid body chemoreceptor and sympathetic overactivity promotes hypertension: a possible new target.

Cardiovascular research·2026
Same author

Gallic Acid Improves Comorbid Chronic Pain and Depression Behaviors by Inhibiting P2X7 Receptor-Mediated Ferroptosis in the Spinal Cord of Rats.

ACS chemical neuroscience·2023
Same author

Imperatorin Improves Obesity-Induced Cardiac Sympathetic Nerve Injury Mediated by P2X4 Receptor in Stellate Sympathetic Ganglion.

International journal of molecular sciences·2023
Same author

Pinocembrin Inhibits P2X4 Receptor-Mediated Pyroptosis in Hippocampus to Alleviate the Behaviours of Chronic Pain and Depression Comorbidity in Rats.

Molecular neurobiology·2022
Same author

Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion.

ACS omega·2022
Same author

Correction to "Contribution of the P2X4 Receptor in Rat Hippocampus to the Comorbidity of Chronic Pain and Depression".

ACS chemical neuroscience·2022

Related Experiment Video

Updated: Feb 5, 2026

Signal Attenuation as a Rat Model of Obsessive Compulsive Disorder
09:29

Signal Attenuation as a Rat Model of Obsessive Compulsive Disorder

Published on: January 9, 2015

15.9K

Evodiamine Attenuates P2X

Yun Xue1,2, Ting Guo3, Lifang Zou1,2

  • 1Department of Physiology, Basic Medical College of Nanchang University, Nanchang, Jiangxi 330006, China.

Oxidative Medicine and Cellular Longevity
|September 14, 2018
PubMed
Summary

Evodiamine reduces P2X7 receptor expression and inflammation in human umbilical vein endothelial cells (HUVECs) exposed to high free fatty acids (FFAs). This suggests potential therapeutic benefits for insulin resistance and type 2 diabetes mellitus (T2DM).

More Related Videos

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
08:46

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Published on: May 18, 2016

18.3K
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
08:34

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Published on: January 8, 2020

7.3K

Related Experiment Videos

Last Updated: Feb 5, 2026

Signal Attenuation as a Rat Model of Obsessive Compulsive Disorder
09:29

Signal Attenuation as a Rat Model of Obsessive Compulsive Disorder

Published on: January 9, 2015

15.9K
Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
08:46

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Published on: May 18, 2016

18.3K
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
08:34

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Published on: January 8, 2020

7.3K

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Cell Biology

Background:

  • Insulin resistance and type 2 diabetes mellitus (T2DM) are global health issues.
  • Elevated free fatty acids (FFAs) are strongly linked to insulin resistance and T2DM.
  • The P2X7 receptor, an ATP-gated ion channel, plays roles in inflammation and cellular responses.

Purpose of the Study:

  • To investigate the role of the P2X7 receptor in high FFA-induced pathological changes in HUVECs.
  • To evaluate the potential protective effects of evodiamine against these changes.

Main Methods:

  • Treatment of HUVECs with high FFAs.
  • Assessment of P2X7 receptor expression (mRNA and protein).
  • Measurement of cytotoxicity, ATP release, reactive oxygen species (ROS) production, nitric oxide (NO) formation, and immune cell adhesion.
  • Analysis of TNF-α expression and ERK 1/2 phosphorylation.

Main Results:

  • High FFAs upregulated P2X7 receptor expression, increased cytotoxicity, ATP release, ROS production, and immune cell adhesion, while decreasing NO formation.
  • Evodiamine suppressed high FFA-induced P2X7 receptor expression.
  • Evodiamine ameliorated cytotoxicity, ATP release, ROS production, and immune cell adhesion.
  • Evodiamine restored NO formation and inhibited P2X7-dependent TNF-α expression and ERK 1/2 phosphorylation.

Conclusions:

  • Evodiamine effectively counteracts high FFA-induced P2X7 receptor upregulation in HUVECs.
  • Evodiamine mitigates oxidative stress and inflammatory responses mediated by P2X7 receptor signaling.
  • These findings highlight evodiamine's potential as a therapeutic agent for conditions associated with high FFAs, insulin resistance, and T2DM.