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Biotoxicological Analyses of Trimeroside from
Marcela Silva Dos Santos1, Juliana da Silva1, Ana Paula Simões Menezes2
1Postgraduate Program of Cellular and Molecular Biology Applied to Health Sciences (PPGBioSaúde), Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
Oxidative Medicine and Cellular Longevity
|September 14, 2018
Summary
Trimeroside from Baccharis trimera exhibits mutagenic and cytotoxic effects in cell assays, despite weak antiproliferative activity. Further research is needed to understand its genotoxicity and folk medicine applications.
Area of Science:
- Phytochemistry
- Toxicology
- Molecular Biology
Background:
- Baccharis trimera is used in folk medicine.
- Oxidative stress mechanisms are linked to DNA damage.
- Trimeroside is a novel nor-monoterpene glycoside isolated from B. trimera.
Purpose of the Study:
- To investigate the biotoxicological effects of trimeroside.
- To determine the genotoxic and mutagenic potential of trimeroside.
- To screen trimeroside's antiproliferative activity against human tumoral cell lines.
Main Methods:
- Isolation of trimeroside and flavonoids using chromatography.
- Genotoxicity and mutagenicity testing via Salmonella/microsome assay, comet assay, and cytokinesis-block micronucleus cytome assay (CBMN-cyt) in HepG2 cells.
- Antiproliferative screening using sulforhodamine B (SRB) assay in human tumoral cell lines.
Main Results:
- Mutagenicity of trimeroside was detected in the TA100 strain with metabolic activation.
- No genotoxic effects were observed in HepG2 cells using the comet assay.
- CBMN-cyt assay indicated cytotoxic and mutagenic effects, with decreased nuclear division index and increased nucleoplasmic bridges at specific concentrations.
- Trimeroside demonstrated weak antiproliferative activity against tested tumoral cell lines.
Conclusions:
- Trimeroside possesses mutagenic and cytotoxic properties.
- The genotoxic effects observed in CBMN-cyt assay warrant further investigation.
- Trimeroside exhibits limited antiproliferative potential, suggesting complex biological activities.
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