Related Experiment Video
Updated: Feb 5, 2026
08:35
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
6.5K
Survival associated alternative splicing events in diffuse large B-cell lymphoma
Rui Zhang1, Peng Lin2, Xia Yang1
1Department of Pathology, First Affiliated Hospital of Guangxi Medical University 6 Shuangyong Road, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
American Journal of Translational Research
|September 14, 2018
Summary
Alternative splicing (AS) variants impact survival in diffuse large B-cell lymphoma (DLBCL). Specific AS events, particularly alternative donor sites, offer prognostic value for DLBCL patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Alternative splicing (AS) is implicated in cancer development.
- Understanding AS variants in diffuse large B-cell lymphoma (DLBCL) is crucial for prognosis.
Purpose of the Study:
- To identify and analyze AS variants associated with overall survival (OS) in DLBCL.
- To explore the correlation between AS events and splicing factors in DLBCL.
Main Methods:
- Analysis of 33,724 AS variant profiles from 48 DLBCL cases.
- Multivariate Cox regression and ROC curve analysis to identify OS-related AS variants.
- Correlation analysis between AS events and 66 splicing factors.
Main Results:
- Ten AS variants were identified as significant predictors of OS in DLBCL.
- Alternative donor (AD) sites in AS events were linked to better outcomes in low-risk DLBCL patients (P=0.0002).
- Splicing factors SF1, HNRNPC, HNRNPD, and HNRNPH3 were significantly associated with OS-related AS variants.
Conclusions:
- Novel prognostic predictors for DLBCL patients were developed based on AS variants.
- Splicing networks involved in DLBCL pathogenesis were mapped for future research.
Related Concept Videos
02:18Alternative RNA Splicing
25.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.2K
02:18Alternative RNA Splicing
5.2K
5.2K
01:32RNA Splicing
60.6K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.6K
01:12Diffusion
219.2K
Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
219.2K
01:21Diffusion
6.4K
Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
6.4K
01:18Survival Curves
719
Survival curves are graphical representations that depict the survival experience of a population over time, offering an intuitive way to track the proportion of individuals who remain event-free at each time point. These curves are widely used in fields such as medicine, public health, and reliability engineering to visualize and compare survival probabilities across different groups or conditions.
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...
The Kaplan-Meier estimator is the most common method for constructing survival curves. This...
719