Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Buffer Effectiveness02:19

Buffer Effectiveness

55.3K
Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.3K
Framing Effects03:26

Framing Effects

8.0K
Information is everywhere and its presentation—such as how and when items are presented—can impact our perceptions and decisions surrounding the info. This broad concept umbrellas framing effects—influences that occur due to the way information is framed in its appearance, whether it’s purely the order or the specific wording of a message. Let’s take a look at numerous ways in which two versions of something can objectively say the same thing, yet we respond in...
8.0K
Biological Effects of Radiation02:59

Biological Effects of Radiation

18.0K
All radioactive nuclides emit high-energy particles or electromagnetic waves. When this radiation encounters living cells, it can cause heating, break chemical bonds, or ionize molecules. The most serious biological damage results when these radioactive emissions fragment or ionize molecules. For example, α and β particles emitted from nuclear decay reactions possess much higher energies than ordinary chemical bond energies. When these particles strike and penetrate matter, they...
18.0K
Effects of feedback01:24

Effects of feedback

1.0K
Feedback in control systems plays a critical role in shaping various operational parameters, extending beyond simple error reduction to influence stability, bandwidth, gain, impedance, and sensitivity. Understanding these effects requires examining a basic feedback system characterized by defined input, output, error, and feedback signals.
Feedback significantly modifies the gain of a control system. The gain of a system without feedback is altered by a factor of one plus GH, where G represents...
1.0K
Self-Awareness and Its Effects01:21

Self-Awareness and Its Effects

316
Self-awareness is a psychological state in which the individual becomes the focal point of their attention. This inward focus transforms the self into an object of contemplation and assessment, influencing how individuals perceive their actions and their alignment with personal and societal standards.Triggers and Contexts for Self-AwarenessSelf-awareness can be activated by external stimuli that make individuals visually or audibly aware of themselves, such as mirrors, cameras, or recordings.
316
Solvating Effects02:12

Solvating Effects

8.8K
An understanding of the solvating effect helps rationalize the relation between solvation and acidity of the compound. In addition, this also explains the relative stability of conjugate bases for compounds with different pKa values. This lesson details, in-depth, the principle of solvating effects. The strength of an acid and the stability of its corresponding conjugate base are determined using pKa values. This observed relationship is a consequence of solvation, which is the interaction...
8.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

New Insight into Pathogenic Variant p.L275P on the Structure and Function of Steroidogenic Acute Regulatory Protein (STAR) Through Molecular Dynamics Simulation.

Advanced biomedical research·2025
Same author

Exosomal miRNA-21-5p and miRNA-21-3p as key biomarkers of myocardial infarction.

Health science reports·2024
Same author

Proof of biased behavior of Normalized Mutual Information.

Scientific reports·2024
Same author

Strict and weak ordinal relations for estimating the criteria weights in Ordinal Priority Approach (OPA).

MethodsX·2023
Same author

Ferulic acid exerts a protective effect against cyclosporine-induced liver injury in rats via activation of the Nrf2/HO-1 signaling, suppression of oxidative stress, inflammatory response, and halting the apoptotic cell death.

Journal of biochemical and molecular toxicology·2023
Same author

Chicoric acid ameliorates palmitate-induced sphingosine 1-phosphate signaling pathway in the PBMCs of patients with newly diagnosed type 2 diabetes.

Journal of diabetes and metabolic disorders·2023
Same journal

Clinical Findings, Antibody Panel and Pathology of Patients with Inflammatory Myopathies in Isfahan Province, Iran.

Advanced biomedical research·2026
Same journal

The Association between Triglycerides to High-density Lipoprotein Cholesterol (TG/HDL-C) Ratio and 10-year Prospective Risk of Type 2 Diabetes Mellitus: Yazd Healthy Heart Project.

Advanced biomedical research·2026
Same journal

Sustained Lung Inflation in Preterm Infants with Signs of Respiratory Distress: A Randomized Controlled Trial.

Advanced biomedical research·2026
Same journal

Dose Prediction Deep Learning-Based Model for VMAT of Prostate Cancer Applying Magnetic Resonance Image (MRI) in Versa HD Linear Accelerator.

Advanced biomedical research·2026
Same journal

Prevalence of Potentially Inappropriate Medication Prescriptions in Older Adults.

Advanced biomedical research·2026
Same journal

Weekly Heart Rate Variability and Training Zone Analysis in U-15 Soccer Players: The Predictive Value of Combined Warm-up Protocols for Optimizing Cardiovascular Monitoring.

Advanced biomedical research·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

Measuring the Behavioral Effects of Intraocular Scatter
05:10

Measuring the Behavioral Effects of Intraocular Scatter

Published on: February 18, 2021

3.9K

Effects of

Amin Mahmoudi1, Keihan Ghatreh Samani2, Effat Farrokhi3

  • 1Department of Biochemistry, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Advanced Biomedical Research
|September 14, 2018
PubMed
Summary
This summary is machine-generated.

Nigella sativa extracts and thymoquinone combat high-fat diet oxidative stress and aid weight loss. These natural compounds positively influence Uncoupling protein-1 (UCP-1) gene and protein expression in brown adipose tissue (BAT).

Keywords:
Antioxidant capacityNigella sativamalondialdehydeobesityuncoupling protein-1

More Related Videos

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
17:35

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

40.5K
State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior
12:38

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior

Published on: December 28, 2010

11.0K

Related Experiment Videos

Last Updated: Feb 5, 2026

Measuring the Behavioral Effects of Intraocular Scatter
05:10

Measuring the Behavioral Effects of Intraocular Scatter

Published on: February 18, 2021

3.9K
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
17:35

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Published on: November 1, 2013

40.5K
State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior
12:38

State-Dependency Effects on TMS: A Look at Motive Phosphene Behavior

Published on: December 28, 2010

11.0K

Area of Science:

  • Metabolic research
  • Nutraceutical science
  • Pharmacology

Background:

  • Uncoupling protein-1 (UCP-1) is a key marker in brown adipose tissue (BAT) for obesity research.
  • High-fat diets induce oxidative stress, impacting metabolic health.
  • Nigella sativa and its active compound thymoquinone (TQ) are investigated for their potential health benefits.

Purpose of the Study:

  • To investigate the effects of TQ, and Nigella sativa hydroalcoholic and hexane extracts on UCP-1 gene expression in BAT.
  • To assess the efficacy of these compounds in mitigating oxidative stress induced by a high-fat diet.
  • To evaluate their impact on body weight and key metabolic markers.

Main Methods:

  • A study involving 50 mice divided into five dietary groups: control, high-fat diet, high-fat diet with hydroalcoholic extract, high-fat diet with hexane extract, and high-fat diet with TQ.
  • Analysis of lipid profile, paraoxonase 1 (PON1) activity, total antioxidant capacity (TAC), and malondialdehyde (MDA) levels.
  • Evaluation of UCP-1 expression at both the gene and protein levels in brown adipose tissue.

Main Results:

  • Mice treated with TQ and Nigella sativa extracts showed significant weight reduction compared to the high-fat diet group.
  • High-fat diet increased MDA (a marker of lipid peroxidation) while decreasing TAC, liver catalase, and PON1 activity.
  • Treatments with TQ and extracts reversed these effects, decreasing MDA and increasing TAC, liver catalase, and PON1 activity.
  • UCP-1 expression was significantly upregulated in the groups treated with hydroalcoholic and hexane extracts.

Conclusions:

  • TQ and Nigella sativa extracts demonstrate a protective and therapeutic effect against high-fat diet-induced oxidative stress.
  • Hydroalcoholic and hexane extracts of Nigella sativa promote weight loss by positively modulating UCP-1 expression at both gene and protein levels.
  • These findings highlight the potential of Nigella sativa as a natural intervention for managing metabolic dysfunction associated with obesity.