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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
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ISSR diversity in
Juan Ubaldo Sánchez-Velázquez1, Ana Ramos-Díaz1
1Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A. C., Sede Sureste, Tablaje Catastral 31264 Km 5.5 Carretera Sierra Papacal-Chuburna Puerto, Parque Científico Tecnológico de Yucatán, CP: 97302 Mérida, Yucatán, Mexico.
Data in Brief
|September 14, 2018
Summary
This study analyzed genetic diversity in Jatropha curcas using ISSR markers. It reveals genetic similarity patterns between parent plants and their offspring, aiding germplasm characterization.
Area of Science:
- Agricultural Science
- Molecular Biology
- Genetics
Background:
- Jatropha curcas is a vital biofuel crop with significant genetic diversity.
- Understanding genetic diversity is crucial for effective breeding programs and germplasm management.
- Previous studies have explored J. curcas genetics, but detailed F1 cross analysis is needed.
Purpose of the Study:
- To assess the genetic diversity within a Jatropha curcas germplasm collection.
- To analyze the genetic similarity between parent accessions and their F1 offspring.
- To evaluate the utility of ISSR markers for J. curcas genetic profiling.
Main Methods:
- Utilized Inter Simple Sequence Repeat (ISSR) molecular markers for genetic analysis.
- Performed Polymerase Chain Reaction (PCR) assays with previously reported primers.
- Calculated Dice similarity coefficients to quantify genetic relatedness between accessions.
Main Results:
- Generated unique genetic profiles for J. curcas accessions and their F1 offspring.
- Quantified genetic similarity, allowing comparison between offspring and parental lines (maternal and paternal sides).
- Demonstrated the effectiveness of ISSR markers in revealing genetic variation within the studied germplasm.
Conclusions:
- ISSR markers are effective tools for evaluating genetic diversity in Jatropha curcas.
- The study provides valuable data for understanding inheritance patterns and selecting superior genotypes.
- Findings support informed decision-making in J. curcas breeding and conservation efforts.
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