Related Experiment Video
Updated: Feb 5, 2026

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture
Published on: July 21, 2020
Positional integration of lung adenocarcinoma susceptibility loci with primary human alveolar epithelial cell
Chenchen Yang1,2,3, Theresa Ryan Stueve1,2,3,4, Chunli Yan1,2,3
1Department of Surgery, University of Southern California, CA 90089, USA.
Aim:
To identify functional lung adenocarcinoma (LUAD) risk SNPs.
Materials & Methods:
Eighteen validated LUAD risk SNPs (p ≤ 5 × 10-8) and 930 SNPs in high linkage disequilibrium (r2 > 0.5) were integrated with epigenomic information from primary human alveolar epithelial cells. Enhancer-associated SNPs likely affecting transcription factor-binding sites were predicted. Three SNPs were functionally investigated using luciferase assays, expression quantitative trait loci and cancer-specific expression.
Results:
Forty-seven SNPs mapped to putative enhancers; 11 located to open chromatin. Of these, seven altered predicted transcription factor-binding motifs. Rs6942067 showed allele-specific luciferase expression and expression quantitative trait loci analysis indicates that it influences expression of DCBLD1, a gene that encodes an unknown membrane protein and is overexpressed in LUAD.
Conclusion:
Integration of candidate LUAD risk SNPS with epigenomic marks from normal alveolar epithelium identified numerous candidate functional LUAD risk SNPs including rs6942067, which appears to affect DCBLD1 expression. Data deposition: Data are provided in GEO record GSE84273.
More Related Videos
14:48Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies
Published on: December 26, 2012
09:27Multicellular Human Alveolar Model Composed of Epithelial Cells and Primary Immune Cells for Hazard Assessment
Published on: May 6, 2020
Related Concept Videos
Root Loci for Positive-Feedback Systems
The construction rules for the root locus in positive feedback systems are similar to those in...
Velocity and Position by Integral Method
Consider an example to calculate the velocity and position from the acceleration function. A motorboat is traveling at a constant velocity of 5.0 m/s when it starts to decelerate to arrive at the dock. Its acceleration is...
Alveoli and Alveolar Ducts
Position-effect Variegation
Magnetic Susceptibility and Permeability
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
Lung Capacity