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Immuno-analytical approach and its application for cardiac disease marker detection
1a Microbiology and Immunology, Faculty of Pharmacy , The British University in Egypt (BUE) , Cairo , Egypt.
Abstract:
Cardiac troponin-I is a promising diagnostic marker for cardiovascular diseases. Troponin-I immunoassays rely on monoclonal antibodies, while polyclonal antibodies, cheaper to manufacture, are uncommonly used. The current study established an immuno-analytical assay using a polyclonal antibody capable of mapping troponin-I antigenic determinant. Proteolytic digestion of troponin-I was performed. Antigenic determinant was assigned by separation of fragments using gel electrophoresis followed by Western blot and high-performance liquid chromatography followed by dot blot. The antigenic determinant region appeared within amino acid sequence 30-90. This robust procedure is suitable for early prognosis of diseases, stratification of patients, and possibly individualized therapy.
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