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Diverse Plasmids Harboring bla

So Yeon Kim1, Kwan Soo Ko1

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|September 14, 2018
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Summary
This summary is machine-generated.

The spread of CTX-M-15-producing Klebsiella pneumoniae ST11 in Asia is not driven by a single clone. Plasmid analysis reveals diverse origins and structures, suggesting recombination rather than clonal dissemination.

Keywords:
CTX-M-15Klebsiella pneumoniaeST11phage shock protein ()prophage

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Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Background:

  • CTX-M-15-producing Klebsiella pneumoniae ST11 isolates are increasingly prevalent in Asian countries.
  • Understanding the dissemination mechanisms of these resistant strains is crucial for public health.

Purpose of the Study:

  • To investigate whether the rise in CTX-M-15-producing Klebsiella pneumoniae ST11 isolates is due to clonal dissemination across Asian countries.
  • To analyze the genetic characteristics of blaCTX-M-15-carrying plasmids in these isolates.

Main Methods:

  • Plasmids were extracted from Klebsiella pneumoniae ST11 isolates collected in Hong Kong, Malaysia, Thailand, and Indonesia.
  • Complete sequencing of blaCTX-M-15-carrying plasmids was performed, including a previously sequenced plasmid from Korea.
  • Plasmid structures, incompatibility groups, antibiotic resistance genes, and prophage genes were analyzed.

Main Results:

  • Plasmids belonged to different incompatibility groups (IncX3, IncF1A, IncFII) and exhibited diverse structures.
  • The number and types of antibiotic resistance genes varied among the sequenced plasmids.
  • All analyzed plasmids harbored cryptic prophage genes, indicating potential roles in plasmid evolution and dissemination.

Conclusions:

  • The prevalence of CTX-M-15-producing Klebsiella pneumoniae ST11 in Asian countries is likely due to diverse plasmid origins and recombination events, not solely clonal dissemination.
  • The findings suggest that the spread of this multidrug-resistant clone is complex and multifactorial.