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Neuroprotective ability of TMV coat protein on rat PC-12 cells and it's in silico study with LRRK2 receptor
Yash Sharma1, Nidhi Srivastava1, Kumud Bala1
1a Center of Medical Biotechnology , Amity Institute of Biotechnology, Amity University Uttar Pradesh , Noida , India.
Objective:
Present study was to observe the neuroprotective ability of TMV coat protein by observing both in vitro studies on Rat PC-12 cells and in silico studies with LRRK2 receptor by molecular docking.
Methods:
TMV coat protein was extracted out from the stem of Nicotiana tabacum and was purified and identified by MALDI-TOF/MS/MS analysis. We confer antioxidant activity of TMV coat protein by enzyme activity like superoxide dismutase (SOD), catalase (CAT) and glutathione-s-transferase (GST) and nonenzyme content by glutathione (GSH) and malondialdehyde (MDA) content. Neuroprotective ability of TMV coat protein was observed by determining the enzyme activity and nonenzyme content in treated cells that were exposed to neurotoxic shock. In silico studies were done in order to observe the molecular docking studies against LRRK2 receptor.
Results:
Antioxidant content was found to be high in TMV coat protein and in treated Rat PC-12 cells as well. Enzyme activity and nonenzyme content were determined and their levels were found to be in increasing level with respect to the volume of 0.2 mg/ml of TMV coat protein. In silico studies revealed the binding efficacy of TMV coat protein with LRRK2 receptor by observing the molecular docking using automated servers.
Conclusion:
From the present study, it was found that TMV coat protein can be utilized as neuroprotective agent and inhibitor of LRRK2 receptor.
