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Updated: Feb 5, 2026

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity
Published on: May 1, 2018
A Sortase A Programmable Phage Display Format for Improved Panning of Fab Antibody Libraries
Henry D Wilson1, Xiuling Li1, Haiyong Peng1
1Department of Immunology and Microbiology, The Scripps Research Institute, Jupiter, FL 33458, USA.
Abstract:
Phage display of combinatorial antibody libraries is a versatile tool in the field of antibody engineering, with diverse applications including monoclonal antibody (mAb) discovery, affinity maturation, and humanization. To improve the selection efficiency of antibody libraries, we developed a new phagemid display system that addresses the complication of bald phage propagation. The phagemid facilitates the biotinylation of fragment of antigen binding (Fab) antibody fragments displayed on phage via Sortase A catalysis and the subsequent enrichment of Fab-displaying phage during selections. In multiple contexts, this selection approach improved the enrichment of target-reactive mAbs by depleting background phage. Panels of cancer cell line-reactive mAbs with high diversity and specificity were isolated from a naïve chimeric rabbit/human Fab library using this approach, highlighting its potential to accelerate antibody engineering efforts and to empower concerted antibody drug and target discovery.
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