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SRPK1 is a poor prognostic indicator and a novel potential therapeutic target for human colorectal cancer

Nan Yi1,2, Mingbing Xiao1,2, Feng Jiang2

  • 1Department of Gastroenterology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, People's Republic of China, weichangchensz@163.com.

Oncotargets and Therapy
|September 15, 2018
PubMed
Abstract

Insights

Serine/arginine protein kinase 1 (SRPK1) is overexpressed in colorectal cancer (CRC), promoting tumor growth and metastasis. Inhibiting SRPK1 suppressed CRC progression, suggesting it as a potential therapeutic target for this disease.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Serine/arginine protein kinase 1 (SRPK1) is a key splicing factor involved in RNA metabolism.
  • High SRPK1 expression is observed in various human cancers.
  • The role of SRPK1 in colorectal cancer (CRC) pathogenesis is not well understood.

Purpose of the Study:

  • To examine SRPK1 expression levels in CRC tissues and cells.
  • To elucidate the functional role and molecular mechanisms of SRPK1 in CRC progression.

Main Methods:

  • Immunohistochemistry, qRT-PCR, and Western blot were used to assess SRPK1 expression.
  • Cell Counting Kit-8, Transwell assays, flow cytometry, and tube formation assays evaluated cell viability, migration, apoptosis, and angiogenesis.
  • Small interfering RNA (siRNA) was employed to inhibit SRPK1.

Main Results:

  • SRPK1 was significantly overexpressed in CRC tissues and cells, correlating with advanced tumor-node-metastasis stage.
  • SRPK1 inhibition led to reduced cell proliferation and migration.
  • Inhibition of SRPK1 significantly increased apoptosis and suppressed angiogenesis in CRC models.

Conclusions:

  • SRPK1 is overexpressed in colorectal cancer and linked to disease progression.
  • SRPK1 inhibition demonstrates anti-cancer effects in CRC.
  • SRPK1 represents a potential prognostic biomarker and therapeutic target for CRC treatment.
Keywords:
angiogenesisapoptosiscolorectal cancergrowthserine/arginine protein kinase 1

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