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Updated: Feb 5, 2026
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Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
Published on: June 29, 2020
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Diagnostic potential of methylated
Jian Zhang1, Zhisen Shen1, Huigao Liu2
1Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, People's Republic of China.
Cancer Management and Research
|September 15, 2018
Summary
Methylated death-associated protein kinase (DAPK) shows a clear association with nasopharyngeal carcinoma (NPC) risk across blood, brushing, and tissue samples. Brushing samples are as effective as tissue samples for monitoring NPC development.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Diagnostics
Background:
- The death-associated protein kinase (DAPK) gene plays a crucial role in apoptosis.
- Aberrant methylation of the DAPK gene is frequently observed in various cancers, including nasopharyngeal carcinoma (NPC).
- The diagnostic utility of DAPK methylation in NPC, particularly in brushing and tissue samples, requires further clarification.
Purpose of the Study:
- To systematically evaluate the diagnostic value of DAPK methylation for nasopharyngeal carcinoma (NPC).
- To compare the effectiveness of DAPK methylation detection in different sample types (blood, brushing, tissue) for NPC diagnosis.
Main Methods:
- A comprehensive meta-analysis was performed.
- The analysis included 17 studies encompassing 386 tissue, 233 brushing, and 296 blood samples.
Main Results:
- A significant association was found between DAPK methylation and an increased risk of NPC across all tested sample types.
- Methylated DAPK in brushing samples demonstrated non-inferior effectiveness compared to tissue samples for NPC monitoring.
- Pooled sensitivity, specificity, and AUC analyses supported the diagnostic potential of DAPK methylation in brushing samples.
Conclusions:
- DAPK methylation serves as a potential biomarker for NPC detection in blood, brushing, and tissue samples.
- DAPK methylation in brushing samples offers a viable and effective alternative to tissue samples for NPC monitoring.
- Further research may solidify the role of DAPK methylation in clinical NPC diagnostics.
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