Related Experiment Video

Updated: Feb 5, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

14.4K

The function of MMP-28/TGF-β induced cell apoptosis in human glioma cells

Xuepeng Wang1, Xi Chen2, Lin Sun3

  • 1Department of Neurosurgery, Affiliated Hospital of Beihua University, Jilin 132000, P.R. China.

Experimental and Therapeutic Medicine
|September 15, 2018
PubMed

Insights

Matrix metalloproteinase (MMP)-28 is downregulated in glioma and influences tumor growth and apoptosis by inducing transforming growth factor (TGF)-β. Low MMP-28 expression correlates with poorer patient survival in glioma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Glioma is a primary brain tumor with complex molecular mechanisms.
  • Matrix metalloproteinase (MMP)-28 is a potential regulator in cancer, but its role in glioma is not fully understood.
  • Transforming growth factor (TGF)-β signaling is implicated in glioma progression.

Purpose of the Study:

  • To investigate the expression status of MMP-28 in glioma.
  • To elucidate the molecular mechanisms of MMP-28 in glioma cells, focusing on its relationship with TGF-β.
  • To assess the impact of MMP-28 on glioma cell growth, apoptosis, and metastasis.

Main Methods:

  • Quantitative polymerase chain reaction (qPCR) for MMP-28 and TGF-β mRNA expression.
  • MTT and Transwell assays for cell proliferation and migration.
  • Annexin V/propidium iodide staining for apoptosis assessment.
  • Western blot analysis for MMP-28 and TGF-β protein levels.

Main Results:

  • MMP-28 and TGF-β expression were significantly downregulated in glioma tissues compared to normal tissues.
  • Lower MMP-28 expression was associated with reduced overall survival and disease-free survival in glioma patients.
  • MMP-28 overexpression upregulated TGF-β, promoting glioma cell growth and inhibiting apoptosis.
  • MMP-28 downregulation suppressed TGF-β, leading to decreased cell growth and increased apoptosis.
  • Inhibition of TGF-β attenuated the effects of MMP-28 on glioma cells.

Conclusions:

  • MMP-28 is downregulated in human glioma and acts as an inducer of TGF-β.
  • MMP-28/TGF-β signaling pathway plays a crucial role in regulating glioma cell proliferation and apoptosis.
  • MMP-28 may serve as a potential therapeutic target or prognostic biomarker for glioma.
Keywords:
glioma cellmatrix metalloproteinase-28transforming growth factor-β

More Related Videos

Spheroid Assay to Measure TGF-β-induced Invasion09:18

Spheroid Assay to Measure TGF-β-induced Invasion

Published on: November 16, 2011

22.5K
In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol08:20

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

21.5K

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.5K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.6K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.5K