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Updated: Feb 5, 2026
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
SSR markers for
David Gutiérrez-Larruscain1,2, Teresa Malvar Ferreras2, M Montserrat Martínez-Ortega1,2
1Departamento de Botánica y Fisiología Vegetal Facultad de Biología University of Salamanca Licenciado Méndez Nieto Ave. E-37007 Salamanca Spain.
Premise Of The Study:
Microsatellite primers were developed for the first time in the genus Filago (Gnaphalieae: Asteraceae). These markers will facilitate low-scale phylogenetic, phylogeographic, and population genetic studies within the genus Filago.
Methods And Results:
Ten pairs of polymorphic microsatellite primers (as well as five pairs of monomorphic primers) were identified and optimized on two species of Filago (F. gaditana and F. carpetana) using a microsatellite-enrichment library method and 454 GS-FLX technique. The polymorphic primers amplified tri- to hexanucleotide repeats and showed one to six alleles per locus for both species. Transferability was performed in 29 samples corresponding to nine representative species of Filago.
Conclusions:
The results indicate the utility of the newly developed markers, which will be useful to delve into the phylogenetic relationships among the taxa within Filago. These microsatellites will enable studies of phylogeographic, reproductive, and genetic variation.
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