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A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

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David Gutiérrez-Larruscain^1,2, Teresa Malvar Ferreras², M Montserrat Martínez-Ortega^1,2

¹Departamento de Botánica y Fisiología Vegetal Facultad de Biología University of Salamanca Licenciado Méndez Nieto Ave. E-37007 Salamanca Spain.

Applications in Plant Sciences

|September 15, 2018

Summary

This summary is machine-generated.

New microsatellite primers for the genus Filago (Asteraceae) are now available. These genetic markers will aid in understanding the evolutionary history and population genetics of Filago species.

Keywords:

Asteraceae Evax Filago microsatellites

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Area of Science:

Botany
Genetics
Evolutionary Biology

Background:

The genus Filago (Asteraceae) lacks established molecular markers for detailed genetic studies.
Understanding phylogenetic and phylogeographic relationships is crucial for conservation and evolutionary insights.

Purpose of the Study:

To develop and characterize novel microsatellite markers for the genus Filago.
To provide essential tools for population genetic, phylogenetic, and phylogeographic research within Filago.

Main Methods:

Microsatellite-enrichment library construction and 454 GS-FLX sequencing were employed.
Primer pairs were designed, optimized, and tested for polymorphism in two Filago species.
Cross-species transferability was assessed across nine Filago species.

Main Results:

Ten polymorphic and five monomorphic microsatellite primer pairs were successfully developed.
Polymorphic markers amplified tri- to hexanucleotide repeats with 1-6 alleles per locus.
Successful transferability across multiple Filago species was demonstrated.

Conclusions:

The developed microsatellite markers are effective and suitable for Filago research.
These markers will facilitate in-depth studies of phylogenetic relationships and genetic diversity.
Future research can now investigate phylogeography and reproductive strategies within the genus.