Related Experiment Video
Updated: Feb 5, 2026

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity
Published on: March 11, 2015
Development and characterization of microsatellite loci for the endemic
Bin-Bin Du1, Ya Zhan1, Jiao-Kun Li2
1School of Life Sciences Central South University Changsha 410013 People's Republic of China.
Premise Of The Study:
Microsatellite primers were developed for the gynodioecious Chinese endemic Thalictrum smithii (Ranunculaceae).
Methods And Results:
Thirty-nine microsatellite primers were developed using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) method. Thirteen microsatellite loci were found to be highly polymorphic after screening 114 specimens (60 hermaphrodite and 54 female) from three T. smithii populations. The number of alleles per locus ranged from three to 13, and the levels of observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.204 to 0.834, respectively. Twenty-six of these primers were polymorphic in T. petaloideum and T. finetii.
Conclusions:
These markers will be useful for examining genetic diversity, polyploidy, and mating system in populations of T. smithii and for guiding study on the evolution of speciation in Thalictrum.
More Related Videos
Related Concept Videos
Root Loci for Positive-Feedback Systems
The construction rules for the root locus in positive feedback systems are similar to those in...
Sustainable Development
Development of the Lymphatic System
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
Language Development
The critical period for language acquisition suggests that the ability to acquire language is at its peak early in life. As people age, this proficiency decreases. Language development begins very...
Development of Immunocompetence
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
Development of the Heart
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart...

