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Zillur Rahman1, Md Anisur Rahman2, Mahamud-Ur Rashid1

  • 1Infectious Diseases Division, International Center for Diarrhoeal Disease Research, Dhaka, Bangladesh.

Frontiers in Public Health
|September 15, 2018
PubMed
Summary

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<i>Vibrio cholerae</i> O1 classical and El Tor toxin-coregulated pilus subunit A (TcpA) epitopes that induce biotype-specific and cross-protective antibodies against bacterial adherence.

Applied and environmental microbiology·2026

This study found that drinking water is a key source for cholera transmission in rural Bangladesh. Interventions promoting safe water are crucial to reduce cholera

Area of Science:

  • Microbiology
  • Epidemiology
  • Public Health

Background:

  • Recurrent cholera outbreaks cause significant illness and death in Bangladesh's estuarine regions.
  • Limited research exists on Vibrio cholerae transmission patterns in Bangladesh.

Purpose of the Study:

  • To characterize Vibrio cholerae O1 isolates from patients, contacts, and water sources in cholera-endemic coastal areas of Bangladesh.
  • To investigate the transmission dynamics of Vibrio cholerae O1 in these regions.

Main Methods:

  • Phenotypic and molecular characterization of Vibrio cholerae isolates (n=56).
  • Molecular fingerprinting using Pulse Field Gel Electrophoresis (PFGE) of Not-I-digested genomic DNA.
  • Phylogenetic analysis using dendrograms.
Keywords:
BangladeshPFGEVibrio choleraecholeraclonal transmissionhousehold contactswater

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Main Results:

  • All characterized Vibrio cholerae isolates were toxigenic, serogroup O1, biotype El Tor (ET), with classical biotype cholera toxin (altered ET).
  • PFGE showed highly homogenous banding patterns, indicating close genetic relatedness between clinical and water-origin isolates.
  • Phylogenetic analysis revealed clonal links within households, implicating water as a significant transmission vehicle.

Conclusions:

  • Toxigenic Vibrio cholerae O1 is transmitted through drinking water in rural Bangladeshi coastal villages.
  • Evidence-based water, sanitation, and hygiene (WASH) interventions are urgently needed to prevent cholera and other enteric infections.