Related Experiment Video
Updated: Feb 5, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Published on: October 15, 2018
An overview on the methods of determining the activity of Indoleamine 2, 3-Dioxygenase 1
Yuandi Zhao1,2,3,4, Bo Wang1,2,3,4, Jinzhi Liu1,2,3,4
1a Collaborative Innovation Center of New Drug Research and Safety , Henan Province , PR China.
Abstract:
Immune escape plays an important hallmark of cancer. The tryptophan-catabolizing enzyme indoleamine 2, 3-dioxygenase 1 (IDO1) is involved in the immune escape mechanism of human tumours, the expression of which is associated with a poor prognosis in cancer and correlated with tumour progression clinically. Therefore, finding the inhibitors targeting IDO1 may be an impellent approach that can reverse the complicated processes in tumour immune escape and induce an antitumor response as well. Here, we summarised and compared some methods of determining the activity of IDO1 and they can be used to screen IDO1 inhibitors as well, including HPLC, fluorescence detection, cell-based assay, NFK GreenScreenTM and absorbance assay. Each determining method possesses special advantages and disadvantages, so we need to have a comprehensive view of these methods and select the most suitable one for further research.
Related Concept Videos
ortho–para-Directing Activators: –CH3, –OH, –⁠NH2, –OCH3
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
Predicting Products: SN1 vs. SN2
With increased substitution on the alkyl halide,...
Sampling Methods: Overview
In analytical chemistry, the choice of...
Preparation of 1° Amines: Hofmann and Curtius Rearrangement Overview
Interfacial Electrochemical Methods: Overview

