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Updated: Feb 5, 2026

Monitoring Protein Adsorption with Solid-state Nanopores
Published on: December 2, 2011
The temporal resolution and single-molecule manipulation of a solid-state nanopore by pressure and voltage
Hengbin Zhang1, Qiang Chen, Yaojun Wu
1Qian Xuesen Laboratory of Space Technology, China Academy of Space Technology, Beijing, 100871, People's Republic of China.
Abstract:
The translocation of DNA molecules through nanopores has attracted wide interest for single-molecule detection. However, the multiple roles of electric fields fundamentally constrain the deceleration and motion control of DNA translocation. In this paper, we show how a single anchored DNA molecule can be manipulated for repeated capture using a transmembrane pressure gradient. Continuously and slowly changing the magnitude of the pressure provided two opposite directions for the force field inside a nanopore, and we observed an anchored DNA molecule entering the nanopore throughout the process from tentative to total entry. The use of both voltage and pressure across a nanopore provides an alternative method to capture, detect and manipulate a DNA molecule at the single-molecule level.
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