Related Experiment Video
Updated: Feb 5, 2026

Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
Circular RNA identified from Peg3 and Igf2r
Bambarendage P U Perera1, Subash Ghimire1, Joomyeong Kim1
1Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, United States of America.
Abstract:
Circular RNA is a newly discovered class of non-coding RNA generated through the back-splicing of linear pre-mRNA. In the current study, we characterized two circular RNAs that had been identified through NGS-based 5'RACE experiments. According to the results, the Peg3 locus contains a 214-nucleotide-long circular RNA, circPeg3, that is detected in low abundance from the neonatal brain, lung and ovary. In contrast, the Igf2r locus contains a group of highly abundant circular RNAs, circIgf2r, showing multiple forms with various exon combinations. In both cases, the expression patterns of circPeg3 and circIgf2r among individual tissues are quite different from their linear mRNA counterparts. This suggests potential unique roles played by the identified circular RNAs. Overall, this study reports the identification of novel circular RNAs specific to mammalian imprinted loci, suggesting that circular RNAs are likely involved in the function and regulation of imprinted genes.
Related Concept Videos
Deformation in a Circular Shaft
RNA Splicing
Stress Concentrations in Circular Shafts
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Uniform Circular Motion
Non-uniform Circular Motion
For example, such...

