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Autophagy in osteoporosis: Relation to oxidative stress

Sara Ezzat1, Manal L Louka1, Zeiad M Zakaria2

  • 1Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.

Journal of Cellular Biochemistry
|September 15, 2018
PubMed

Insights

Autophagy-related gene 7 (Atg7) and superoxide dismutase 2 (SOD2) gene expression are significantly decreased in osteoporosis. These genes are involved in osteoporosis pathogenesis and may offer future therapeutic targets.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pathogenesis of Osteoporosis

Background:

  • Impaired autophagy and oxidative stress are linked to various diseases.
  • Autophagy and oxidative stress play roles in cellular health and disease development.

Purpose of the Study:

  • Investigate the role of autophagy-related gene 7 (Atg7) and superoxide dismutase 2 (SOD2) in osteoporosis.
  • Evaluate the gene expression and enzyme activity of Atg7 and SOD2 in osteoporotic patients.

Main Methods:

  • Quantitative real-time PCR (SYBR green) for Atg7 and SOD2 gene expression.
  • Colorimetric assay for SOD2 enzyme activity.
  • Comparison between an osteoporotic group (n=26) and an osteoporosis-free group (n=14).

Main Results:

  • Both Atg7 and SOD2 gene expression were significantly lower (P < 0.01) in the osteoporotic group.
  • SOD2 enzyme activity showed no significant difference between groups.
  • A significant positive correlation was found between Atg7 and SOD2 gene expression in both groups.
  • Atg7 expression correlated positively with vitamin D levels and BMI in osteoporotic patients.

Conclusions:

  • Atg7 and SOD2 gene expression are involved in osteoporosis pathogenesis.
  • The findings suggest potential therapeutic strategies targeting Atg7 and SOD2 for osteoporosis treatment.
Keywords:
autophagyautophagy-related gene 7 (Atg7)osteoporosisoxidative stresssuperoxide dismutase 2 (SOD2)

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