Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Factors Influencing Attraction I: Proximity01:22

Factors Influencing Attraction I: Proximity

277
Proximity plays a fundamental role in shaping interpersonal attraction by increasing opportunities for interaction and fostering familiarity. Research consistently demonstrates that individuals are more likely to form social bonds with those who are physically closer to them, whether in residential settings, workplaces, or educational institutions. This effect is largely driven by the increased frequency of encounters, which facilitates the development of friendships and romantic...
277
Subviral Agents01:29

Subviral Agents

567
Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
567
Air-entraining Agents01:27

Air-entraining Agents

273
Air-entraining agents improve the durability and workability of concrete in climates with frequent freezing and thawing. These agents prevent cracks by introducing small air bubbles into the mix, creating spaces accommodating water expansion when temperatures drop. The air-entraining agents lower the surface tension of water, forming stable, small air bubbles. This method is more effective than having accidental large voids, as the intentional, smaller, and evenly distributed air voids improve...
273
Masking and Demasking Agents01:19

Masking and Demasking Agents

3.6K
EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
3.6K
Spasmolytic Agents: Chemical Classification01:29

Spasmolytic Agents: Chemical Classification

1.3K
Spasmolytic agents are drugs used to alleviate muscle spasms and spasticity. They can be categorized into different chemical groups based on their mechanisms of action. Centrally acting spasmolytics primarily affect the spinal cord, while others directly target skeletal muscle cells.
A major class of centrally acting spasmolytics is the α2-agonist, such as tizanidine. These drugs bind to α2-adrenoceptors, inhibiting the release of the excitatory neurotransmitter glutamate. They also...
1.3K
Oral Hypoglycemic Agents: Glinides01:06

Oral Hypoglycemic Agents: Glinides

671
Repaglinide (Prandin) and Nateglinide (Starlix), known as glinides, are oral insulin secretagogues that stimulate insulin release from pancreatic β cells by closing the ATP-sensitive potassium channels (KATP channel). Repaglinide controls insulin release from pancreatic β cells by managing potassium efflux. It shares two binding sites with sulfonylureas and also has a unique site, indicating overlapping mechanisms of action. With a rapid onset and a 4-7 hour duration, it effectively...
671

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vaccination elicits HIV broadly neutralizing antibodies in primates.

Nature·2026
Same author

Translating Innovation to Clinic: End-to-End Bioprocess Development and cGMP Manufacturing of N332-GT5 HIV Vaccine Candidate for First-in-Human Trials HVTN144.

bioRxiv : the preprint server for biology·2026
Same author

Diverse germline-targeting HIV Env immunogens select for distinct mutations in the same knock-in mice B cell receptors.

Nature communications·2026
Same author

Development and Deployment of an Activity-Based N-Glycan Hydrolase Probe Suitable for Droplet-Based Screening.

Journal of the American Chemical Society·2026
Same author

Chemoenzymatic Synthesis of N-Linked Glycan Receptors of H1N1 Influenza Virus on Human Airway Epithelial Cells.

Journal of the American Chemical Society·2026
Same author

Fatal human H3N8 influenza virus has a moderate pandemic risk.

PLoS pathogens·2026

Related Experiment Video

Updated: Feb 5, 2026

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
09:31

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Published on: April 15, 2017

19.0K

Proximity Ligation-Based Fluorogenic Imaging Agents for Neuraminidases.

Zhizeng Gao1,2, Andrew J Thompson3, James C Paulson3

  • 1Department of Chemistry, University of British Columbia, Vancouver, British Columbia, V6T 1Z1, Canada.

Angewandte Chemie (International Ed. in English)
|September 15, 2018
PubMed
Summary
This summary is machine-generated.

Researchers developed novel neuraminidase-imaging probes for visualizing enzyme activity. These proximity ligation reagents specifically detect influenza virus infections in cells, offering advantages over traditional antibodies.

Keywords:
fluorogenic probeshistological imaginginfluenza virusneuraminidase

More Related Videos

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
11:27

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Published on: December 4, 2016

10.6K
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.9K

Related Experiment Videos

Last Updated: Feb 5, 2026

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
09:31

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals

Published on: April 15, 2017

19.0K
Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging
11:27

Preparation and In Vitro Characterization of Dendrimer-based Contrast Agents for Magnetic Resonance Imaging

Published on: December 4, 2016

10.6K
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
09:18

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay

Published on: October 20, 2018

7.9K

Area of Science:

  • Biochemistry
  • Cell Biology
  • Virology

Background:

  • Neuraminidase activity is crucial in cellular processes, viral infections, and cancer.
  • Existing methods for visualizing neuraminidase activity have limitations.

Purpose of the Study:

  • To develop novel neuraminidase-imaging probes.
  • To create reagents for detecting influenza virus infections.
  • To offer alternatives to anti-neuraminidase antibodies.

Main Methods:

  • Proximity ligation assay development.
  • Design of neuraminidase-sensitive probes releasing fluorophores.
  • Testing of probes for influenza virus detection in mammalian cells.

Main Results:

  • A new class of neuraminidase-imaging probes was successfully synthesized.
  • The probes function as proximity ligation reagents, releasing reactive fluorophores.
  • An influenza virus-specific probe was developed, enabling detection of viral infections.
  • Probes demonstrated specificity and potential advantages over antibody-based methods.

Conclusions:

  • Novel neuraminidase-imaging probes offer a new tool for studying enzyme activity.
  • These probes can specifically detect influenza virus infections.
  • The reagents provide a promising alternative to existing histological probes.