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Updated: Feb 5, 2026

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples
Published on: October 2, 2020
Ethylenediamine assisted functionalization of self-organized poly (d, l-lactide-co-glycolide) patterned surface to
Namita Jaiswal1, Abhiram Hens2, Manosree Chatterjee1
1Material Processing & Microsystem Laboratory, CSIR - Central Mechanical Engineering Research Institute, Durgapur 713209, India; Department of Biotechnology, National Institute of Technology Durgapur, Durgapur 713209, India.
Abstract:
Protein functionalized micro-scale patterned structures are developed using a biocompatible polymer PLGA (poly (d, l-lactide-co-glycolide)) via thin film dewetting and by step-wise chemical conjugations with EDA (ethylenediamine) and anti-EpCAM (Epithelial Cell Adhesion Molecule) antibodies to target the epithelial cell adhesion molecules of cancer cells. The effectiveness of such protein functionalized patterned surface is checked through cell isolation process using blood samples spiked with different cancer cells such as MCF-7, A549, MDA-MB-231. An efficient capture yield of 92% is obtained with MCF-7 cells over a two hour incubation time. The study demonstrates the effects of cell concentration and incubation time on the binding of cancer cells to the modified patterned surfaces. For the first time, a simple and inexpensive method is reported to fabricate functionalized PLGA patterned surface for an efficient isolation of cancer cells from diluted blood samples. The method shows the potential to be used as an effective platform for the development of an improved circulating tumor cell (CTC) isolation device from the clinical blood sample.
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