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Updated: Feb 5, 2026

Bioassays for Monitoring Insecticide Resistance
Published on: December 30, 2010
Resistance of
Bussarakam Chuppava1, Birgit Keller2, Amr Abd El-Wahab3
1Institute for Animal Nutrition, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, D-30173 Hanover, Germany. bussarakam.chuppava@tiho-hannover.de.
Antibiotic resistance in livestock is a major concern. Double enrofloxacin treatment significantly reduced susceptible Escherichia coli, regardless of flooring type, while environmental exposure had no effect.
Area of Science:
- Veterinary Medicine
- Microbiology
- Animal Science
Background:
- Antibiotic resistance in livestock poses a significant challenge to animal health and food safety.
- Understanding factors influencing resistance development is crucial for effective control strategies.
Purpose of the Study:
- To evaluate the impact of enrofloxacin treatment and environmental exposure on antibacterial resistance in commensal Escherichia coli in turkeys.
- To investigate the influence of different flooring systems on resistance development.
Main Methods:
- A total of 720 turkeys were used across three trials.
- Four flooring designs (litter, heated, partially slatted, fully slatted with sand bath) were tested.
- Commensal Escherichia coli isolates from cloacal swabs and manure were analyzed for enrofloxacin and ampicillin resistance using broth microdilution (MIC).
Main Results:
- Double enrofloxacin treatment significantly decreased susceptible Escherichia coli across all flooring types.
- Environmental exposure simulation did not significantly affect resistance levels.
- Flooring design did not demonstrate a significant impact on resistance.
- Ampicillin-resistant Escherichia coli were detected, despite ampicillin not being administered.
Conclusions:
- Enrofloxacin administration is a key driver of reduced susceptibility in Escherichia coli.
- Flooring type and environmental exposure simulation appear to have minimal influence on the development of resistance in this context.
- The presence of ampicillin resistance warrants further investigation into potential cross-resistance or other contributing factors.
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