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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
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Lin Chen1, Tiantian Cao2, Jin Zhang3

  • 1State Key Laboratory of Rice Biology & Ministry of Agriculture Key Lab of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. chenlin88@126.com.

International Journal of Molecular Sciences
|September 16, 2018
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Summary

The rice gibberellin (GA) receptor gene OsGID1 mediates resistance to the brown planthopper (BPH). Overexpressing OsGID1 in rice plants enhances defense mechanisms, reducing BPH populations and improving plant resistance.

Keywords:
Nilaparvata lugensOsGID1gibberellinherbivore-induced plant defensesrice

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Area of Science:

  • Plant Science
  • Molecular Biology
  • Entomology

Background:

  • Gibberellins (GAs) are crucial for plant growth, development, and pathogen defense.
  • The role of GA signaling in plant defense against herbivore attack is not well understood.

Purpose of the Study:

  • To investigate the function of the rice GA receptor gene OsGID1 in plant defense against the brown planthopper (BPH).
  • To elucidate the molecular mechanisms underlying GA-mediated resistance to BPH in rice.

Main Methods:

  • Cloning and characterization of the rice GA receptor gene OsGID1.
  • Analysis of OsGID1 expression under various stress conditions (BPH damage, mechanical wounding, salicylic acid, jasmonic acid).
  • Overexpression of OsGID1 (oe-GID1) in rice and assessment of defense-related hormone levels (SA, ethylene) and gene expression (WRKY).
  • Laboratory bioassays and field studies to evaluate BPH preference, egg hatching, and population density on wild type (WT) and oe-GID1 plants.
  • Measurement of lignin levels in oe-GID1 plants.

Main Results:

  • OsGID1 expression was induced by BPH damage, wounding, and salicylic acid (SA).
  • Overexpression of OsGID1 (oe-GID1) reduced SA and H₂O₂ levels but increased ethylene levels in response to BPH.
  • Gravid BPH females showed reduced feeding and egg-laying preference on oe-GID1 plants.
  • Egg hatching rates and BPH population densities (adults and nymphs) were significantly lower on oe-GID1 plants in both lab and field conditions.
  • Increased lignin levels and decreased expression of SA-pathway WRKY genes were observed in oe-GID1 plants.

Conclusions:

  • The OsGID1-mediated GA pathway plays a positive role in enhancing rice resistance to BPH.
  • OsGID1 influences plant defense signaling by modulating SA and ethylene pathways and affecting WRKY gene expression.
  • Increased lignin deposition, potentially mediated by the GA pathway, contributes to BPH resistance in rice.