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Developmental changes in cardiac sarcoplasmic reticulum in sheep.

L Mahony, L R Jones

    The Journal of Biological Chemistry
    |November 15, 1986
    PubMed
    Summary

    Fetal sheep hearts show altered sarcoplasmic reticulum (SR) function, with reduced calcium (Ca2+) pump density but higher pump efficiency, potentially explaining lower contractile reserve in newborns.

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    Area of Science:

    • Cardiovascular Physiology
    • Developmental Biology
    • Molecular Cardiology

    Background:

    • Fetal and newborn sheep myocardium exhibit higher contractility but reduced reserve compared to adults.
    • Sarcoplasmic reticulum (SR) Ca2+ transport is crucial for cardiac muscle function and may differ developmentally.

    Purpose of the Study:

    • To investigate the functional and compositional differences in cardiac SR between fetal and maternal sheep.
    • To elucidate the role of SR Ca2+ transport in the altered contractile state of fetal myocardium.

    Main Methods:

    • Isolation and functional analysis of cardiac SR vesicles from fetal and maternal sheep.
    • Quantification of Ca2+ uptake, Ca2+-dependent ATPase activity, and protein composition (Ca2+ pump, phospholamban, calsequestrin, nitrendipine binding sites).

    Main Results:

    • Fetal SR vesicles showed a 60% decrease in Ca2+ accumulation and Ca2+ pump density, but only a 30% decrease in Ca2+-dependent ATPase activity.
    • Fetal SR Ca2+ pumps exhibited a 1.6-fold higher turnover rate.
    • Fetal SR vesicles demonstrated increased passive Ca2+ permeability and higher content of calsequestrin and nitrendipine-binding sites, with decreased phospholamban.

    Conclusions:

    • Developmental changes in cardiac SR, including altered Ca2+ pump kinetics and potentially increased junctional SR, may contribute to the reduced myocardial contractile reserve in fetal sheep.
    • These findings highlight significant adaptations in SR Ca2+ handling during cardiac development.

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