Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thao T Nguyen1,2, Grzegorz Sabat1, Michael R Sussman3,2

  • 1From the Biotechnology Center and.

The Journal of Biological Chemistry
|September 16, 2018
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activating adaptor-like sequences in pericentrin mediate its transport by dynein.

bioRxiv : the preprint server for biology·2026
Same author

Deep Learning for Path Loss Prediction at 7 GHz in Urban Environment.

IEEE access : practical innovations, open solutions·2026
Same author

Thymic Atypical Carcinoid Mimicking Recurrent Type A Thymoma on Frozen Section: A Diagnostic Pitfall Resolved by Intraoperative Imprint Cytology.

Cureus·2026
Same author

Structure of the chromoprotein ovorubin from the golden apple snail (Pomacea canaliculata).

Protein science : a publication of the Protein Society·2025
Same author

Plant Proteomics: New Insights Into the Green World Through Advanced Mass Spectrometry.

Molecular & cellular proteomics : MCP·2025
Same author

Impact of metritis and systemic antibiotic treatment on the biology and morphology of the bovine uterus at one month postpartum†.

Biology of reproduction·2025
Same journal

YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

The Journal of biological chemistry·2026
Same journal

ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

The Journal of biological chemistry·2026
Same journal

PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

The Journal of biological chemistry·2026
Same journal

Saturated cardiolipins are potent disruptors of inner mitochondrial membrane structure and function.

The Journal of biological chemistry·2026
Same journal

Phosphate release from myosin Va occurs after the initial powerstroke but before the secondary powerstroke associated with ADP-release.

The Journal of biological chemistry·2026
Same journal

Epigenetic silencing of miR-141 via core promoter methylation is associated with short-term bladder cancer progression.

The Journal of biological chemistry·2026
See all related articles

Researchers identified how the C-terminal regulatory domain of plant proton pumps interacts with the N-terminal actuator domain. This finding suggests a "head-to-tail" monomer organization essential for proton pump function and regulation.

Area of Science:

  • Plant molecular biology
  • Biochemistry
  • Membrane transport

Background:

  • P-type proton-pumping ATPases are crucial for plant growth, generating proton-motive force.
  • Previous studies lacked structural details on the C-terminal regulatory domain's interaction with catalytic domains.
  • The precise interaction sites for regulation remained unknown, despite genetic insights.

Purpose of the Study:

  • To investigate the interaction between the C-terminal regulatory domain and catalytic domains of plant proton pumps.
  • To provide direct chemical evidence for amino acid residues involved in this interaction.
  • To elucidate the structural organization of these essential membrane proteins.

Main Methods:

  • In vivo cross-linking using a photoreactive unnatural amino acid (p-benzoylphenylphenylalanine).
Keywords:
Arabidopsis thalianaH+-ATPasemass spectrometry (MS)protein cross-linkingprotein-protein interaction

Related Experiment Videos

  • Tandem mass spectrometry (MS/MS) to identify cross-linked peptides.
  • Analysis of protein oligomerization states (dimers, trimers, hexamers).

    • Main Results:

    • Direct evidence was obtained for the interaction between the C-terminal regulatory domain and the N-terminal actuator domain.
    • Specific amino acid residues involved in this intermolecular interaction were identified.
    • The findings support a model of "head-to-tail" ATPase monomer organization.

    Conclusions:

    • The C-terminal regulatory domain interacts with the N-terminal actuator domain of another ATPase molecule.
    • This intermolecular interaction is crucial for proton pump regulation and function.
    • The proposed model explains the observed homopolymerization (dimers, trimers, hexamers) of the proton pump in the membrane.