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Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) analysis of micro-droplets offers a sensitive, high-throughput method for elemental quantification in biological samples. This validated technique overcomes matrix effects and ensures accurate analysis of low-volume samples.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Micro-droplet analysis using LA-ICP-MS is emerging for biological samples.
  • It offers advantages for low-volume samples and native concentrations compared to traditional methods.
  • Matrix effects on residue deposition and signal intensity require characterization.

Purpose of the Study:

  • To develop and validate a high-throughput LA-ICP-MS method for elemental quantification in micro-droplets.
  • To investigate and characterize matrix effects on elemental signal intensity.
  • To assess the method's accuracy, specificity, and reproducibility in biological matrices.

Main Methods:

  • Validated high-throughput method using LA-ICP-MS imaging and matrix-matched external calibrants.
  • Analysis of micro-droplet residue across the entire area to account for heterogeneous distribution.
  • Examination of matrix effects from proteins, salts, and reagents used in biochemical assays.

Main Results:

  • Matrix composition causes concentration-dependent signal enhancement/suppression for carbon.
  • High sodium content induces space-charge-like suppression effects on high masses.
  • Method accuracy confirmed with certified serum standards and solution nebulisation ICP-MS.

Conclusions:

  • The developed LA-ICP-MS method is rapid, flexible, and transferable to various biological matrices.
  • It enables high-throughput analysis of low-volume samples with sensitivity comparable to traditional ICP-MS.
  • The imaging approach mitigates analytical uncertainty from heterogeneous droplet residues.