N-(aminobutyl)-N-(ethylisoluminol) functionalized Fe-based metal-organic frameworks with intrinsic mimic peroxidase activity for sensitive electrochemiluminescence mucin1 determination
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Updated: Feb 5, 2026

Synthesis and Characterization of Functionalized Metal-organic Frameworks
Published on: September 5, 2014
Zili Wang1, Xinya Jiang1, Ruo Yuan1
1Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Abstract:
Herein, N-(4-aminobutyl)-N-(ethylisoluminol) functionalized Fe-based metal-organic frameworks (ABEI/MIL-101(Fe)) with intrinsic mimic peroxidase activity was synthesized and utilized as highly efficient ECL indicator to construct sensitive immunosensor for mucin1 (MUC1) detection. Firstly, compared with the traditional method for ABEI immobilization, the proposed strategy could successfully achieve the highly efficient ABEI immobilization as ABEI coupled 2-aminoterephhalic acid was used as organic bridge ligand. Moreover, the ABEI/MIL-101(Fe) containing the same luminescent group as luminol could emit strong light when using hydrogen peroxide (H2O2) as a co-reactant. It is worth noting that the ECL signal of ABEI/MIL-101(Fe) could be greatly heightened due to the intrinsic mimic peroxidase activity of ABEI/MIL-101(Fe) that could accelerate the decomposition of H2O2 and produce considerable numbers of reactive oxygen radicals to participate in the ECL reaction of ABEI. The fabricated ECL immunosensor displayed a low detection limit of 1.6 fg mL-1 for MUC1, indicating that the synthesized ABEI/MIL-101(Fe) with intrinsic mimic peroxidase activity may be applied to realize ultrasensitive detection of other biomarkers in early diagnosis.
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