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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy

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Switchable Fluorophores for Single-Molecule Localization Microscopy.

Honglin Li, Joshua C Vaughan

Chemical Reviews

|September 18, 2018

Summary

This summary is machine-generated.

Super-resolution microscopy, particularly single-molecule localization microscopy (SMLM), relies on switchable fluorophores. This review covers major SMLM fluorophore classes and imaging strategies.

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Area of Science:

* Biophysics and advanced optical imaging.

Background:

* Super-resolution fluorescence microscopy has rapidly advanced biological and materials science research.
* Single-molecule localization microscopy (SMLM) is a key super-resolution technique.
* SMLM's efficacy depends on precisely controlling fluorophore states (on/off).

Observation:

* Switchable fluorophores are crucial for enabling controlled blinking in SMLM.
* Various chemical, photochemical, and binding mechanisms facilitate fluorophore switching.
* A comprehensive review of SMLM fluorophore classes is presented.

Findings:

* Detailed review of major SMLM fluorophore categories.
* Strategies for specimen labeling are discussed.
* Considerations for multichannel and live-cell imaging are addressed.

Implications:

* Guides researchers in selecting and utilizing fluorophores for SMLM.
* Highlights potential challenges and future research directions in SMLM technology.
* Facilitates advancements in high-resolution biological imaging.