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Updated: Feb 5, 2026

gP2S, an Information Management System for CryoEM Experiments
Published on: June 10, 2021
CryoEM of bacterial secretion systems
Romain Kooger1, Piotr Szwedziak1, Désirée Böck1
1Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, CH-8093 Zürich, Switzerland.
Abstract:
The need for bacteria to interact with their environment has driven the evolution of elaborate secretion systems. By virtue of their function, secretion systems are macromolecular complexes associated with the cell envelope and therefore inherently difficult to study by conventional structural biology techniques. Cryo-electron microscopy (cryoEM) has become an invaluable technique to study large membrane-embedded complexes and led to major advances in the mechanistic understanding of secretion systems. CryoEM comprises of two main modalities, namely single particle analysis and tomography. Here, we review how detailed structures retrieved by single particle analysis combine elegantly with tomography experiments in which the secretion systems are observed in their native cellular context.
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