Related Experiment Video
Updated: Feb 5, 2026

Intravenous Injections in Neonatal Mice
Published on: November 11, 2014
Stromal interaction molecule 1 is required for neonatal testicular development in mice
Cong Shen1, Ke Zhang2, Jun Yu3
1Center for Reproduction and Genetics, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, China; State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, China.
Abstract:
Stromal interaction molecule 1 (STIM1) is a transmembrane endoplasmic reticulum protein, and it serves as a Ca2+ sensor and activator of store-operated Ca2+ entry (SOCE). We have previously identified STIM1 in the proteome profile of mice neonatal testes, revealing STIM1 to be associated with neonatal testicular development. Here, to further explore the location and function of STIM1 in mice testes, we studied the effect of Stim1 gene knockdown on neonatal testicular development by testicular culture. Our results revealed that STIM1 was primarily located in Sertoli cells. Knockdown of Stim1 gene using morpholino in neonatal testis caused the mislocation of Sertoli cells and loss of germ cells, which were associated with the aberrant reactive oxygen species (ROS) activation, while inhibition of ROS could partly rescue the phenotypes caused by Stim1 gene knockdown. In conclusion, our study suggests that STIM1 can maintain neonatal testicular development by inhibiting ROS activation.
Related Concept Videos
Piaget's Stage 1 of Cognitive Development
Exploration...
Energy-requiring Steps of Glycolysis
Requirements for Human Life
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
Molecules and Compounds
Proteins: Dietary Sources and Requirements
Oxygen Requirements and Growth Patterns

