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Updated: Feb 5, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization
Ning Chen1, Weixia Li1, Shuchao Wu2
1Department of Chemistry, Zhejiang University, Xixi Campus, Hangzhou 310028, People's Republic of China.
Abstract:
A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM H3PO4 and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0 mg l-1 with a correlation coefficient of 0.9997. The limit of qualification (S/N = 10) and limit of detection (S/N = 3) were 9.7 and 2.9 µg l-1, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.
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