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Related Experiment Videos

Chan Hee Moon1, Ji Yun Lee1, Eun Soon Kim1

  • 1Department of Ophthalmology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea.

International Journal of Ophthalmology
|September 19, 2018
PubMed
Summary
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Topically administered 7-taurocholic acid conjugated low-molecular weight heparin (LHT7) shows high ocular biodistribution in neovascularized corneas for 4 hours. Reapplication may be necessary to maintain therapeutic effects in corneal neovascularization models.

Area of Science:

  • Ophthalmology
  • Pharmacology
  • Biomedical Imaging

Background:

  • Corneal neovascularization (CoNV) poses a significant challenge in ophthalmology.
  • Effective topical drug delivery to neovascularized corneal tissue is crucial for treatment.
  • Understanding drug biodistribution is key to optimizing therapeutic strategies.

Purpose of the Study:

  • To evaluate the ocular biodistribution and clearance of topically applied 7-taurocholic acid conjugated low-molecular weight heparin (LHT7).
  • To assess the drug's behavior in a mouse model of corneal neovascularization using in vivo optical imaging.
  • To determine the optimal application frequency for LHT7 in treating CoNV.

Main Methods:

  • Corneal neovascularization was induced in BALB/c mice via corneal sutures.
Keywords:
corneal neovascularizationin vivo optical imaginglow-molecular weight heparinocular biodistribution

Related Experiment Videos

  • A near-infrared fluorescent probe (Cy5.5) labeled LHT7 (LHT7-Cy5.5) was topically instilled.
  • In vivo optical imaging tracked LHT7-Cy5.5 distribution in neovascularized corneas over 6 hours.

    • Main Results:

    • LHT7-Cy5.5 demonstrated significantly higher intensity in the inferior cornea (neovascularized area) compared to the superior cornea at 5 minutes, 2 hours, and 4 hours post-instillation.
    • The drug concentration in the inferior cornea decreased to 42.9% of its initial value by 6 hours.
    • Control groups using free Cy5.5 showed no significant difference in intensity between corneal regions.

    Conclusions:

    • Topically administered LHT7 exhibits substantial biodistribution within corneal neovascularization areas for up to 4 hours.
    • The findings suggest that LHT7 may require reapplication within 4 hours to maintain therapeutic levels.
    • In vivo optical imaging is a valuable tool for assessing ocular drug delivery and biodistribution in preclinical models.