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Reconstruct Human Retinoblastoma In Vitro
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Reconstruct Human Retinoblastoma In Vitro

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Expression of multidrug-resistance associated proteins in human retinoblastoma treated by primary enucleation

Li-Juan Tang1, Li-Jun Zhou1, Wen-Xin Zhang1

  • 1State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China.

International Journal of Ophthalmology
|September 19, 2018
PubMed
Abstract

Insights

Retinoblastoma (RB) intrinsically expresses multidrug-resistance proteins like glutathione-S-transferase π (GSTπ) and P-glycoprotein (P-gp). GSTπ shows strong correlation with well-differentiated RB tumors, suggesting it as a potential chemotherapy target.

Area of Science:

  • Ophthalmology
  • Oncology
  • Molecular Biology

Background:

  • Retinoblastoma (RB) is a common intraocular malignancy in children.
  • Multidrug-resistance (MDR) proteins, including GSTπ, P-gp, and LRP, are implicated in treatment failure.
  • Understanding MDR protein expression in RB is crucial for developing effective therapeutic strategies.

Purpose of the Study:

  • To investigate the expression of GSTπ, P-gp, and LRP in retinoblastoma.
  • To correlate the expression of these MDR proteins with histopathological features of RB.
  • To identify potential therapeutic targets for RB treatment.

Main Methods:

  • Analysis of 42 retinoblastoma specimens obtained via primary enucleation.
  • Immunohistochemical staining to detect GSTπ, P-gp, and LRP expression.
  • Correlation of protein expression with tumor differentiation and invasion status.

Main Results:

  • GSTπ was expressed in 92.86% of RB cases, with 100% positivity in well-differentiated tumors.
  • P-gp and GSTπ co-expression was observed in 71.42% of RBs.
  • No significant correlation was found between P-gp, GSTπ, or LRP expression and tumor invasion into ocular layers or the optic nerve.

Conclusions:

  • Retinoblastoma intrinsically expresses GSTπ, P-gp, and LRP.
  • GSTπ expression is strongly associated with well-differentiated RB, suggesting a role in tumor differentiation.
  • GSTπ emerges as a potential novel therapeutic target for retinoblastoma chemotherapy.
Keywords:
P-glycoproteinglutathione-S-transferase πmultidrug-resistance proteinsretinoblastomavault protein lung resistance protein

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