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Biomarker identification of thyroid associated ophthalmopathy using microarray data.

Hong-Bin Yang1, Jie Jiang1, Lu-Lu Li2

  • 1Department of Ophthalmology, the First Affiliated Hospital of Harbin Medical University, Harbin 150080, Heilongjiang Province, China.

International Journal of Ophthalmology
|September 19, 2018
PubMed
Summary
This summary is machine-generated.

This study identified key genes like TPX2 and MKI67 involved in thyroid-associated ophthalmopathy (TAO) pathogenesis. MKI67 shows potential as a biomarker for TAO, offering new insights into disease mechanisms.

Keywords:
biomarkercell cyclemicroarraythyroid associated ophthalmopathy

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Area of Science:

  • Ophthalmology
  • Genomics
  • Molecular Biology

Background:

  • Thyroid-associated ophthalmopathy (TAO) is an autoimmune condition affecting the eye orbit.
  • Understanding TAO pathogenesis is crucial for developing effective treatments.
  • Identifying molecular players and biomarkers can improve TAO diagnosis and management.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying TAO.
  • To identify potential diagnostic and prognostic biomarkers for TAO.

Main Methods:

  • Gene expression profiling of TAO patients versus controls using the Gene Expression Omnibus database (GSE9340).
  • Identification of differentially expressed genes (DEGs) using R and limma package.
  • Functional enrichment analysis with DAVID and protein-protein interaction network construction using STRING and BinGO.

Main Results:

  • 861 DEGs were identified between TAO and control groups.
  • Key genes including TPX2, CDCA5, PRC1, KIF23, and MKI67 were significantly associated with TAO and enriched in cell cycle processes.
  • MKI67 demonstrated a strong correlation with TAO, and GTF2F1, SMC3, USF1, ZNF263 were predicted as transcription factors.

Conclusions:

  • TPX2, CDCA5, PRC1, and KIF23 are implicated in TAO pathogenesis through cell cycle regulation.
  • MKI67 is a potential biomarker for TAO.
  • SMC3 and ZNF263 may function as transcription factors in TAO progression.