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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
Published on: March 15, 2019
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Whole-genome sequence data and analysis of a
Yanping Xie1, Yiping He1, Sandeep Ghatak2
1Molecular Characterization of Foodborne Pathogens Research Unit, Eastern Regional Research Center, Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 600 East Mermaid Lane, Wyndmoor, PA 19038, USA.
Data in Brief
|September 19, 2018
Summary
The genome of Staphylococcus aureus SJTUF_J27, isolated from Chinese seaweed, reveals antimicrobial resistance and virulence genes. This study provides valuable genomic data for understanding S. aureus diversity and potential public health implications.
Area of Science:
- Microbiology
- Genomics
- Bioinformatics
Background:
- Staphylococcus aureus is a significant human pathogen.
- Understanding the genomic diversity of S. aureus is crucial for public health.
- Marine environments can harbor bacterial strains with unique genetic traits.
Purpose of the Study:
- To report the complete genome sequence of S. aureus SJTUF_J27 isolated from seaweed.
- To identify virulence factors and antimicrobial resistance genes within this strain.
- To perform comparative genomic analysis with other S. aureus strains.
Main Methods:
- Whole-genome sequencing of S. aureus SJTUF_J27.
- Bioinformatic analysis to identify coding sequences, RNAs, and genes.
- Phylogenetic analysis using genome-wide single nucleotide polymorphisms (SNPs).
- Comparative genomic evaluation using BLAST Ring Image Generator (BRIG).
Main Results:
- The genome size is 2.8 Mbp with 32.9% G+C content, containing 2614 coding sequences and 77 RNAs.
- Identified antimicrobial resistance genes (fluoroquinolone, beta-lactams, fosfomycin, mupirocin, trimethoprim, aminocoumarin) and the egc enterotoxin cluster.
- Genes for metal-binding proteins and heavy metal resistance were detected.
- Phylogenetic analysis placed SJTUF_J27 within the S. aureus species, with comparative analysis highlighting genomic differences from food-isolated strains.
Conclusions:
- The S. aureus SJTUF_J27 genome harbors significant virulence and antimicrobial resistance genes, suggesting potential pathogenicity.
- The presence of heavy metal resistance genes indicates adaptation to specific environmental conditions.
- This genomic data contributes to the understanding of S. aureus evolution and its presence in diverse ecological niches.
- The study provides a valuable reference genome for future research on S. aureus.
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