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Updated: Feb 5, 2026
20:23
A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
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Glycodendron-rhenium complexes as luminescent probes for lectin sensing
Alessandro Palmioli1, Monica Panigati, Anna Bernardi
1Department of Chemistry, University of Milano, via Golgi 19, 20133 Milano, Italy. alessandro.palmioli@unimib.it.
Organic & Biomolecular Chemistry
|September 19, 2018
Summary
Novel luminescent probes using dinuclear rhenium(i) complexes were developed. These probes target specific proteins (lectins) and exhibit enhanced light emission upon binding, acting as "switch-on" tools for biological sensing.
Area of Science:
- Inorganic Chemistry
- Bioconjugation Chemistry
- Supramolecular Chemistry
Background:
- Carbohydrate-binding proteins (lectins) play crucial roles in biological processes.
- Developing sensitive and specific probes for lectin detection is vital for biological research and diagnostics.
- Rhenium(i) complexes offer unique photophysical properties suitable for luminescent probe development.
Purpose of the Study:
- To design, synthesize, and characterize novel glycoconjugate luminescent probes.
- To utilize a multivalent platform with diverse carbohydrate moieties for targeting specific lectins.
- To explore the potential of rhenium(i) luminophores for sensitive protein sensing applications.
Main Methods:
- Synthesis of dinuclear rhenium(i) complexes functionalized with carbohydrate moieties (glucose, galactose, mannose).
- Characterization of the synthesized glycoconjugates using spectroscopic and analytical techniques.
- Investigation of the binding interactions between the glycoconjugates and specific lectins (ConA, PNA) in aqueous media.
Main Results:
- The synthesized glycoconjugates were non-luminescent in aqueous solutions.
- Specific binding of the glycoconjugates to target lectins (ConA and PNA) was confirmed.
- Binding events triggered a significant enhancement in the emission intensity of the rhenium(i) probes, demonstrating a "switch-on" effect.
Conclusions:
- Dinuclear rhenium(i)-based glycoconjugates function as effective "switch-on" luminescent probes.
- These probes exhibit specific binding to lectins, leading to enhanced luminescence.
- The developed probes hold promise for applications in biological sensing and imaging.
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