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Expression analysis of limb element markers during mouse embryonic development.

Alexandra Rafipay1, Amanda L R Berg1, Lynda Erskine1

  • 1School of Medicine, Medical Sciences and Nutrition, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|September 19, 2018
PubMed
Summary
This summary is machine-generated.

This study maps key gene expression patterns during embryonic mouse limb development. These findings provide essential benchmarks for analyzing limb development in mutant mouse models.

Keywords:
Alcian Blue/Alizarin RedGene expressioncartilageforelimbhindlimbjointlimb developmentmusclenervetendonvascular

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Anatomy

Background:

  • Limited data exist on limb element and tissue marker expression during normal mouse limb development.
  • Few studies compare upper and lower limb development across key embryonic stages.
  • Establishing normal developmental patterns is crucial for analyzing mutant mouse models.

Purpose of the Study:

  • To present expression patterns of key markers during embryonic mouse limb patterning.
  • To describe nerve development and cartilage formation during limb development.
  • To provide methods for assessing normal and abnormal limb development.

Main Methods:

  • Analysis of gene expression patterns for Gdf5, Scleraxis, MyoD1, and Cadherin5 (Cdh5).
  • Utilizing anti-neurofilament staining for developing nerves in forelimbs and hindlimbs.
  • Describing cartilage formation and progression during embryonic limb development.

Main Results:

  • Detailed expression patterns of joint, tendon, ligament, muscle, and blood vessel markers are presented.
  • Developing nerves in both forelimbs and hindlimbs were visualized using anti-neurofilament staining.
  • Cartilage formation and its progression were described throughout key developmental phases.

Conclusions:

  • This study establishes key morphological markers and methods for evaluating limb development.
  • The presented data serve as a critical reference for normal limb development.
  • These methods aid in the assessment of both normal and abnormal limb development in mice.