Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Separable function of platelet release reaction and clot retraction.

Y Yatomi, M Higashihara, A Tanabe

    Biochemical and Biophysical Research Communications
    |October 15, 1986
    PubMed
    Summary

    Amiloride and cytochalasin B differentially affect platelet activation. These findings show that platelet serotonin release and clot retraction are distinct processes.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effectiveness of post-trastuzumab deruxtecan treatments and incidence of interstitial lung disease in HER2-positive metastatic breast cancer: a real-world, observational cohort study.

    ESMO open·2025
    Same author

    Joint instability causes catabolic enzyme production in chondrocytes prior to synovial cells in novel non-invasive ACL ruptured mouse model.

    Osteoarthritis and cartilage·2022
    Same author

    Effect of human adipose-derived mesenchymal stem cell conditioned medium on musculoskeletal pain.

    European review for medical and pharmacological sciences·2022
    Same author

    The difference in joint instability affects the onset of cartilage degeneration or subchondral bone changes.

    Osteoarthritis and cartilage·2021
    Same author

    Pathophysiological characteristics of non-alcoholic steatohepatitis-like changes in cholesterol-loaded type 2 diabetic rats.

    Physiological research·2018
    Same author

    The RPMI-1640 vitamin mixture promotes bovine blastocyst development in vitro and downregulates gene expression of TXNIP with epigenetic modification of associated histones.

    Journal of developmental origins of health and disease·2017

    Area of Science:

    • Biochemistry
    • Hematology
    • Cell Biology

    Background:

    • Platelet activation involves complex cellular processes.
    • Serotonin release and clot retraction are key platelet functions.

    Purpose of the Study:

    • To investigate the effects of amiloride and cytochalasin B on platelet serotonin release and clot retraction.
    • To determine if these two platelet activation processes can be functionally separated.

    Main Methods:

    • Dose-dependent inhibition of platelet serotonin release by amiloride.
    • Assessment of amiloride's effect on clot retraction at varying platelet concentrations.
    • Evaluation of cytochalasin B's impact on both serotonin release and clot retraction.

    Main Results:

    Related Experiment Videos

    • Amiloride inhibited platelet serotonin release in a dose-dependent manner.
    • Amiloride (1mM) accelerated clot retraction at decreased platelet concentrations.
    • Cytochalasin B accelerated serotonin release but inhibited clot retraction.

    Conclusions:

    • Platelet serotonin release and clot retraction can be functionally separated.
    • Amiloride and cytochalasin B exhibit distinct modulatory effects on platelet functions.