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Updated: Feb 5, 2026

Building a Better Mosquito: Identifying the Genes Enabling Malaria and Dengue Fever Resistance in A. gambiae and A. aegypti Mosquitoes
Published on: July 4, 2007
The Development of Cytogenetic Maps for Malaria Mosquitoes
Gleb N Artemov1, Vladimir N Stegniy2, Maria V Sharakhova3,4
1Laboratory of Ecology, Genetics and Environmental Protection, Tomsk State University, 36 Lenin Avenue, 634050 Tomsk, Russia. g-artemov@mail.ru.
Abstract:
Anopheline mosquitoes are important vectors of human malaria. Next-generation sequencing opens new opportunities for studies of mosquito genomes to uncover the genetic basis of a Plasmodium transmission. Physical mapping of genome sequences to polytene chromosomes significantly improves reference assemblies. High-resolution cytogenetic maps are essential for anchoring genome sequences to chromosomes as well as for studying breakpoints of chromosome rearrangements and chromatin protein localization. Here we describe a detailed pipeline for the development of high-resolution cytogenetic maps using polytene chromosomes of malaria mosquitoes. We apply this workflow to the refinement of the cytogenetic map developed for Anopheles beklemishevi.
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