Related Experiment Video

Updated: Feb 5, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.3K

Involvement of NOS2 Activity on Human Glioma Cell Growth, Clonogenic Potential, and Neurosphere Generation

Paola Palumbo1, Francesca Lombardi2, Giuseppe Siragusa3

  • 1Department of Life, Health & Environmental Sciences, University of L'Aquila, Building Delta 6, Coppito, 67100 L'Aquila, Italy. paola.palumbo@univaq.it.

International Journal of Molecular Sciences
|September 20, 2018
PubMed

Insights

Inhibiting nitric oxide synthase 2 (NOS2) with 1400W suppressed glioma cell proliferation, migration, and neurosphere formation. This highlights NOS2 as a potential therapeutic target for aggressive brain tumors like glioblastoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Neuroscience

Background:

  • Aberrant nitric oxide synthase 2 (NOS2) expression is linked to human malignant tumors, including glioblastoma.
  • Glioblastoma is an aggressive brain tumor with poor prognosis and limited treatment options.

Purpose of the Study:

  • To investigate the effect of the specific NOS2 inhibitor 1400W on human glioma cells.
  • To assess the role of the NOS2/NO system in glioma cell proliferation, migration, and stemness.

Main Methods:

  • Western blotting for NOS2 expression.
  • Nitrite level determination for nitric oxide (NO) production.
  • Cell proliferation, clonogenic potential, migration, and neurosphere generation assays.
  • Treatment with 1400W on glioblastoma cell lines and primary glioma cells.

Main Results:

  • NOS2 expression was confirmed in glioma cells and primary cultures, and overexpressed in derived neurospheres.
  • 1400W treatment significantly inhibited glioma cell proliferation, colony formation, and migration.
  • 1400W also reduced neurosphere generation ability in glioma cells.

Conclusions:

  • The NOS2/NO system plays a crucial role in glioma cell growth, invasion, and stemness.
  • Inhibiting NOS2 with 1400W demonstrates therapeutic potential against glioblastoma.
  • NOS2/NO system activity may serve as a prognostic factor for glioma malignancy and recurrence.
Keywords:
1400W NOS2 inhibitorNOS2clonogenic potentialhuman glioma cellsneurosphere generationtumor cell proliferation and migration

More Related Videos

Generation of Human CD40-activated B cells13:27

Generation of Human CD40-activated B cells

Published on: October 16, 2009

15.7K
A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells05:29

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

Published on: March 28, 2021

3.2K

Related Concept Videos

Cell Potential and Free Energy02:58

Cell Potential and Free Energy

Thermodynamics of a Redox Reaction
Thermodynamics is the branch of physics dealing with the relationship between heat and other forms of energy. In an electrochemical cell, chemical energy is converted into electrical energy.
Thus, a link can be predicted between cell potential, free energy change, and the equilibrium constant for the reaction. Cell potential can also be measured as the oxidant or the reducing strength, and similar acid-base strength measures are reflected in equilibrium...
46.6K
Thermodynamics: Chemical Potential and Activity01:10

Thermodynamics: Chemical Potential and Activity

The effective concentration of a species in a solution can be expressed precisely in terms of its activity. Activity considers the effect of electrolytes present in the vicinity of the species of interest and depends on the ionic strength of the solution. The activity of a species is expressed as the product of molar concentration and the activity coefficient of the species.
The thermodynamic equilibrium constant is more accurately defined in terms of activity rather than concentration.
1.7K
Generation of Action Potential in Skeletal Muscles01:24

Generation of Action Potential in Skeletal Muscles

Every cell in the body maintains a membrane potential due to an uneven distribution of positive and negative charges across its plasma membrane. The membrane potential is measured in millivolts and quantifies the difference in charge across the membrane.
Like neurons, muscle cells are also regarded as excitable due to their capacity to change in response to stimuli, primarily due to voltage-gated ion channels embedded in their plasma membranes, which get activated by alterations in the...
8.8K
Meristems and Plant Growth02:36

Meristems and Plant Growth

Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
49.5K
Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.5K
Potential Energy00:52

Potential Energy

The energy stored by a structure and location of matter in space is called potential energy. For instance, raising a kettlebell changes its spatial location and increases its potential energy. Similarly, a stretched rubber band contains potential energy which, under certain conditions, can be converted into other forms of energy, such as kinetic energy.
Chemical bonds that form attractive forces between atoms also contain potential energy, called chemical energy. When a chemical reaction...
42.7K